Anti-MRP8 + MRP14 antibody [5.5] (ab20222)

Product name

Anti-MRP8 + MRP14 antibody [5.5]
See all MRP8 + MRP14 primary antibodies
Description

Mouse monoclonal [5.5] to MRP8 + MRP14
Host species

Mouse
Tested applications

Suitable for: IP, WB, IHC-Frmore details
Species reactivity

Reacts with: Human
Immunogen

Tissue/ cell preparation, acute monocytic leukaemia cells (Human).
Positive control
- Neutrophil lysate (particularly neutrophils from spleen)

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Constituent: PBS
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

5.5
Isotype

IgG1
Research areas

Our Abpromise guarantee covers the use of ab20222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration. Predicted molecular weight MRP8 10.8 kDa, MRP14 13 kDa.
IHC-Fr		Use at an assay dependent concentration.

Relevance

MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
Cellular localization

Cytoplasmic and Nuclear
Database links
- Entrez Gene: 6279 Human
- Entrez Gene: 6280 Human
- Omim: 123885 Human
- Omim: 123886 Human
- SwissProt: P05109 Human
- SwissProt: P06702 Human
- Unigene: 112405 Human
- Unigene: 416073 Human
Alternative names
- CAGA antibody
- CAGB antibody
- Calgranulin A antibody
- Calgranulin B antibody
- Calprotectin L1L subunit antibody
- CFAG antibody
- CP10 antibody
- Cystic fibrosis antigen antibody
- Leukocyte L1 complex light chain antibody
- Macrophage L1 protein Calprotectin antibody
- Migration inhibitory factor related protein 14 antibody
- Migration inhibitory factor related protein 8 antibody
- MRP-14 antibody
- MRP-8 antibody
- P14 antibody
- P8 antibody
- S100 calcium binding protein A8 antibody
- S100 calcium binding protein A9 antibody
- S100A8 antibody
- S100A9 antibody
- Urinary stone protein band A antibody
see all

Wen H et al. Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma. Nat Med 24:154-164 (2018). WB . Read more (PubMed: 29291352) »
Wang Y et al. Platelet-derived S100 family member myeloid-related protein-14 regulates thrombosis. J Clin Invest 124:2160-71 (2014). WB, IHC ; Mouse . Read more (PubMed: 24691441) »

Publishing research using ab20222? Please let us know so that we can cite the reference in this datasheet.

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Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Rabbit Cell lysate - whole cell (Fibroblasts)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

20 µg

Specification

Fibroblasts

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Ms. Asmaa Alsousi

Verified customer

Submitted Dec 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"