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    mrp8-mrp14-antibody-55-ab20222.pdf

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Cell Biology Cell Cycle Cell differentiation
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Anti-MRP8 + MRP14 antibody [5.5] (ab20222)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Key features and details

  • Mouse monoclonal [5.5] to MRP8 + MRP14
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Protein
Recombinant Human MRP8 + MRP14 protein (ab130945)
Primary
Product image
Biotin Anti-MRP8 + MRP14 antibody (ab51863)

View more associated products

Overview

  • Product name

    Anti-MRP8 + MRP14 antibody [5.5]
    See all MRP8 + MRP14 primary antibodies
  • Description

    Mouse monoclonal [5.5] to MRP8 + MRP14
  • Host species

    Mouse
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation, acute monocytic leukaemia cells (Human).

  • Positive control

    • Neutrophil lysate (particularly neutrophils from spleen)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    5.5
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human MRP8 + MRP14 protein (ab130945)

Target

  • Relevance

    MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

    • Entrez Gene: 6279 Human
    • Entrez Gene: 6280 Human
    • Omim: 123885 Human
    • Omim: 123886 Human
    • SwissProt: P05109 Human
    • SwissProt: P06702 Human
    • Unigene: 112405 Human
    • Unigene: 416073 Human
    • Alternative names

      • CAGA antibody
      • CAGB antibody
      • Calgranulin A antibody
      • Calgranulin B antibody
      • Calprotectin L1L subunit antibody
      • CFAG antibody
      • CP10 antibody
      • Cystic fibrosis antigen antibody
      • Leukocyte L1 complex light chain antibody
      • Macrophage L1 protein Calprotectin antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor related protein 8 antibody
      • MRP-14 antibody
      • MRP-8 antibody
      • P14 antibody
      • P8 antibody
      • S100 calcium binding protein A8 antibody
      • S100 calcium binding protein A9 antibody
      • S100A8 antibody
      • S100A9 antibody
      • Urinary stone protein band A antibody
      see all

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab20222? Please let us know so that we can cite the reference in this datasheet.

    ab20222 has been referenced in 4 publications.

    • Wen H  et al. Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma. Nat Med 24:154-164 (2018). WB . PubMed: 29291352
    • Wang Y  et al. Platelet-derived S100 family member myeloid-related protein-14 regulates thrombosis. J Clin Invest 124:2160-71 (2014). WB, IHC ; Mouse . PubMed: 24691441
    • Hessian PA & Fisher L The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9). Antibody recognition, epitope definition and the implications for structure. Eur J Biochem 268:353-63 (2001). PubMed: 11168370
    • Hogg N  et al. Monoclonal antibody 5.5 reacts with p8,14, a myeloid molecule associated with some vascular endothelium. Eur J Immunol 19:1053-61 (1989). PubMed: 2666142

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-MRP8 + MRP14 antibody [5.5]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Cell lysate - whole cell (Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    Fibroblasts
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Ms. Asmaa Alsousi

    Verified customer

    Submitted Dec 20 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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