Anti-Calprotectin (S100A8 + S100A9) antibody [B314.1 (MAC 387)] (ab130234)
Key features and details
- Mouse monoclonal [B314.1 (MAC 387)] to Calprotectin (S100A8 + S100A9)
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog, Pig, Monkey, Baboon
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Calprotectin (S100A8 + S100A9) antibody [B314.1 (MAC 387)]
See all Calprotectin (S100A8 + S100A9) primary antibodies -
Description
Mouse monoclonal [B314.1 (MAC 387)] to Calprotectin (S100A8 + S100A9) -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog, Pig, Monkey, Baboon -
Immunogen
Tissue, cells or virus corresponding to Human S100A9+S100A8.
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Positive control
- Human tonsil, lymph Node or spleen tissues.
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General notes
Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Please see notes section. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, Dialysed media, Fetal calf serum, 0.24% Tris, 0.12% Boric Acid, Carrier protein, Carrier polysaccharides -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
B314.1 (MAC 387) -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
Prolonged fixation in buffered formalin can destroy the epitope. |
Notes |
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IHC-P
Use at an assay dependent concentration. Prolonged fixation in buffered formalin can destroy the epitope. |
Target
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Cellular localization
S100A9: Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. S100A8: Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway. -
Database links
- Entrez Gene: 20202 Mouse
- Entrez Gene: 94195 Rat
- SwissProt: P27005 Mouse
- SwissProt: P31725 Mouse
- SwissProt: P50115 Rat
- SwissProt: P50116 Rat
- Unigene: 2128 Mouse
- Unigene: 6703 Rat
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab130234 has been referenced in 1 publication.
- Hrabar J et al. Interplay between proinflammatory cytokines, miRNA, and tissue lesions in Anisakis-infected Sprague-Dawley rats. PLoS Negl Trop Dis 13:e0007397 (2019). PubMed: 31091271