Key features and details
- Mouse monoclonal [B314.1 (MAC 387)] to MRP8 + MRP14
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog, Pig, Monkey, Baboon
- Isotype: IgG1
Product nameAnti-MRP8 + MRP14 antibody [B314.1 (MAC 387)]
See all MRP8 + MRP14 primary antibodies
DescriptionMouse monoclonal [B314.1 (MAC 387)] to MRP8 + MRP14
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog, Pig, Monkey, Baboon
Affinity purified monocyte membrane preparation.
- Human tonsil, lymph Node or spleen tissues.
General notesDilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Please see notes section.
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, Dialysed media, Fetal calf serum, 0.24% Tris, 0.12% Boric Acid, Carrier protein, Carrier polysaccharides
Concentration information loading...
PurityImmunogen affinity purified
Clone numberB314.1 (MAC 387)
Our Abpromise guarantee covers the use of ab130234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Prolonged fixation in buffered formalin can destroy the epitope.|
RelevanceMRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
Cellular localizationCytoplasmic and Nuclear
- CAGA antibody
- CAGB antibody
- Calgranulin A antibody
ab130234 has been referenced in 1 publication.
- Hrabar J et al. Interplay between proinflammatory cytokines, miRNA, and tissue lesions in Anisakis-infected Sprague-Dawley rats. PLoS Negl Trop Dis 13:e0007397 (2019). PubMed: 31091271