Overview

  • Product name

    Anti-MRPL16 antibody [EPR14351-63]
    See all MRPL16 primary antibodies
  • Description

    Rabbit monoclonal [EPR14351-63] to MRPL16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MRPL16 aa 100-250. The exact sequence is proprietary.
    Database link: Q9NX20

  • Positive control

    • HepG2, 293, HeLa and Human fetal heart lysates; Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Component of the large subunit of mitochondrial ribosome.
  • Sequence similarities

    Belongs to the ribosomal protein L16P family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 39S ribosomal protein L16 antibody
    • 39S ribosomal protein L16 mitochondrial antibody
    • L16mt antibody
    • mitochondrial antibody
    • Mitochondrial ribosomal protein L16 antibody
    • MRP-L16 antibody
    • Mrpl16 antibody
    • PNAS 111 antibody
    • RM16_HUMAN antibody
    see all

Images

  • All lanes : Anti-MRPL16 antibody [EPR14351-63] (ab181840) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-MRPL16 antibody [EPR14351-63] (ab181840) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MRPL16 with ab181840 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

ab181840 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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