Recombinant Anti-MRPL16 antibody [EPR14351-63] (ab181840)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14351-63] to MRPL16
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MRPL16 antibody [EPR14351-63]
See all MRPL16 primary antibodies -
Description
Rabbit monoclonal [EPR14351-63] to MRPL16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, 293, HeLa and Human fetal heart lysates; Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14351-63 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 28 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 28 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Component of the large subunit of mitochondrial ribosome. -
Sequence similarities
Belongs to the ribosomal protein L16P family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 54948 Human
- Omim: 611829 Human
- SwissProt: Q9NX20 Human
- Unigene: 530734 Human
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Alternative names
- 39S ribosomal protein L16 antibody
- 39S ribosomal protein L16 mitochondrial antibody
- L16mt antibody
see all
Images
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All lanes : Anti-MRPL16 antibody [EPR14351-63] (ab181840) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
All lanes : Anti-MRPL16 antibody [EPR14351-63] (ab181840) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MRPL16 with ab181840 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181840 has not yet been referenced specifically in any publications.