Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14351] to MRPL16
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-MRPL16 antibody [EPR14351]
See all MRPL16 primary antibodies
DescriptionRabbit monoclonal [EPR14351] to MRPL16
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MRPL16 aa 100-250. The exact sequence is proprietary.
Database link: Q9NX20
- HepG2, HeLa and Human fetal heart lysate. Human colon tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 28 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the large subunit of mitochondrial ribosome.
Sequence similaritiesBelongs to the ribosomal protein L16P family.
- Information by UniProt
- 39S ribosomal protein L16 antibody
- 39S ribosomal protein L16 mitochondrial antibody
- L16mt antibody
All lanes : Anti-MRPL16 antibody [EPR14351] (ab181834) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Blocking/ Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin embedded Human colon tissue sections labeling MRPL16 using ab181834 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab181834 has not yet been referenced specifically in any publications.