Recombinant Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7578(B)] to MRPL28
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MRPL28 antibody [EPR7578(B)]
See all MRPL28 primary antibodies -
Description
Rabbit monoclonal [EPR7578(B)] to MRPL28 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal kidney tissue lysates JAR cell lysates HepG2 whole cell lysate (ab7900) A549 cell lysates 293T cell lysates A549 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7578(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Tissue specificity
Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue. -
Sequence similarities
Belongs to the ribosomal protein L28P family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 10573 Human
- Entrez Gene: 68611 Mouse
- Entrez Gene: 497876 Rat
- Omim: 604853 Human
- SwissProt: Q13084 Human
- SwissProt: Q9D1B9 Mouse
- Unigene: 513230 Human
- Unigene: 273102 Mouse
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Alternative names
- 39S ribosomal protein L28 antibody
- 39S ribosomal protein L28 mitochondrial antibody
- 39S ribosomal protein L28 mitochondrial precursor antibody
see all
Images
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All lanes : Anti-MRPL28 antibody [EPR7578(B)] (ab126719) at 1/1000 dilution
Lane 1 : Fetal kidney tissue lysate
Lane 2 : JAR cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : 293T (Human embryonic kidney epithelial cell) cell lysate
Predicted band size: 30 kDa -
ab126719, at 1/250 dilution, stains MRPL28 in A549 cells by immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126719 has been referenced in 1 publication.
- Schneider K et al. Protein Stability Buffers the Cost of Translation Attenuation following eIF2a Phosphorylation. Cell Rep 32:108154 (2020). PubMed: 32937139