Recombinant
RabMAb

Recombinant Anti-MRPL28 antibody [EPR7578(B)] (ab126719)

Overview

  • Product name

    Anti-MRPL28 antibody [EPR7578(B)]
    See all MRPL28 primary antibodies
  • Description

    Rabbit monoclonal [EPR7578(B)] to MRPL28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human MRPL28 (Q13084).

  • Positive control

    • Fetal kidney cell lysates JAR cell lysates HepG2 whole cell lysate (ab7900) A549 cell lysates 293T cell lysates A549 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IP 1/100 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Tissue specificity

      Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.
    • Sequence similarities

      Belongs to the ribosomal protein L28P family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • 39S ribosomal protein L28 antibody
      • 39S ribosomal protein L28 mitochondrial antibody
      • 39S ribosomal protein L28 mitochondrial precursor antibody
      • HGNC6756 antibody
      • L28mt antibody
      • MAAT 1 antibody
      • MAAT1 antibody
      • Melanoma antigen p15 antibody
      • Melanoma associated antigen recognised by cytotoxic T lymphocytes antibody
      • Melanoma associated antigen recognized by T lymphocytes antibody
      • Melanoma-associated antigen recognized by T-lymphocytes antibody
      • MGC8499 antibody
      • mitochondrial antibody
      • Mitochondrial ribosomal protein L28 antibody
      • MRP L28 antibody
      • MRP-L28 antibody
      • MRPL 28 antibody
      • mrpl28 antibody
      • P15 antibody
      • RM28_HUMAN antibody
      see all

    Images

    • All lanes : Anti-MRPL28 antibody [EPR7578(B)] (ab126719) at 1/1000 dilution

      Lane 1 : Fetal kidney cell lysates
      Lane 2 : JAR cell lysates
      Lane 3 : HepG2 cell lysates
      Lane 4 : A549 cell lysates
      Lane 5 : 293T cell lysates

      Predicted band size: 30 kDa

    • ab126719, at 1/250 dilution, stains MRPL28 in A549 cells by immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126719 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Loading amount
    6.5 µg
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Sample
    Human Cell lysate - whole cell (HepG2)
    Specification
    HepG2
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 26 2015

    Answer

    Thank you for your reply. I am glad I could help you choose the best antibody for your experiment.

    We do not have any distributor in Norway, you can order directly from us. Please let me know if you need a proforma and I will be happy to send it to you.

    If not, you can order directly through mailto:orders@abcam.com indicating your billing and shipping address as well as a valid VAT number.

    If you need further assistance, please do not hesitate to contact us back.

    Read More

    Answer

    Thank you for contacting us.

    There are four antibodies in our catalogue suitable for WB in either one of the mentioned species.

    Product ab126719 is a rabbit monoclonal that has been validated by WB in Human, Mouse and Rat, you can have a look at the blot image on its datasheet:

    https://www.abcam.com/MRPL28-antibody-EPR7578B-ab126719.html

    Alternatively, https://www.abcam.com/MRPL28-antibody-ab89030.html and https://www.abcam.com/MRPL28-antibody-ab15871.html are also suitable for WB in Human:

    https://www.abcam.com/MRPL28-antibody-ab89030.html

    https://www.abcam.com/MRPL28-antibody-ab15871.html

    And ab98922 has been tested in Human, but due to sequence homology it is also expected to react with Mouse and Rat:

    https://www.abcam.com/MRPL28-antibody-ab98922.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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