Recombinant Anti-MRPL30 antibody [EPR12502] (ab179819)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12502] to MRPL30
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-MRPL30 antibody [EPR12502]
See all MRPL30 primary antibodies -
Description
Rabbit monoclonal [EPR12502] to MRPL30 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human MRPL30. The exact sequence is proprietary.
Database link: Q8TCC3 -
Positive control
- 293T, A375 and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12502 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa. |
Target
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Relevance
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene. -
Database links
- Entrez Gene: 51263 Human
- Entrez Gene: 107734 Mouse
- Entrez Gene: 301352 Rat
- Omim: 611838 Human
- SwissProt: Q8TCC3 Human
- SwissProt: Q9D7N6 Mouse
- SwissProt: P0C2C1 Rat
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Alternative names
- 39S ribosomal protein L28, mitochondrial antibody
- 39S ribosomal protein L30, mitochondrial antibody
- L28MT antibody
see all
Images
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All lanes : Anti-MRPL30 antibody [EPR12502] (ab179819) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A375 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab179819 has not yet been referenced specifically in any publications.