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RecombinantRabMAb

Recombinant Anti-MRPL30 antibody [EPR12502] (ab179819)

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Western blot - Anti-MRPL30 antibody [EPR12502] (ab179819)
  • Anti-MRPL30 antibody [EPR12502] (ab179819)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12502] to MRPL30
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-MRPL30 antibody [EPR12502]
    See all MRPL30 primary antibodies
  • Description

    Rabbit monoclonal [EPR12502] to MRPL30
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human MRPL30. The exact sequence is proprietary.
    Database link: Q8TCC3

  • Positive control

    • 293T, A375 and HeLa cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12502
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Mito. Translation

Associated products

  • Alternative Versions

    • Anti-MRPL30 antibody [EPR12502] - BSA and Azide free (ab250121)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Relevance

      Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene.
    • Database links

      • Entrez Gene: 51263 Human
      • Entrez Gene: 107734 Mouse
      • Entrez Gene: 301352 Rat
      • Omim: 611838 Human
      • SwissProt: Q8TCC3 Human
      • SwissProt: Q9D7N6 Mouse
      • SwissProt: P0C2C1 Rat
      • Alternative names

        • 39S ribosomal protein L28, mitochondrial antibody
        • 39S ribosomal protein L30, mitochondrial antibody
        • L28MT antibody
        • L30MT antibody
        • Mitochondrial ribosomal protein L30 antibody
        • MRP L28 antibody
        • MRP L30 antibody
        • MRPL28 antibody
        • MRPL28M antibody
        • RPML28 antibody
        see all

      Images

      • Western blot - Anti-MRPL30 antibody [EPR12502] (ab179819)
        Western blot - Anti-MRPL30 antibody [EPR12502] (ab179819)
        All lanes : Anti-MRPL30 antibody [EPR12502] (ab179819) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : A375 cell lysate
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 19 kDa

      • Anti-MRPL30 antibody [EPR12502] (ab179819)
        Anti-MRPL30 antibody [EPR12502] (ab179819)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab179819? Please let us know so that we can cite the reference in this datasheet.

      ab179819 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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