Anti-MRPL34 antibody (ab151055)
Key features and details
- Rabbit polyclonal to MRPL34
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MRPL34 antibody -
Description
Rabbit polyclonal to MRPL34 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment, corresponding to amino acids 16-92 of Human MRPL34 (UniProt ID: Q9BQ48).
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Positive control
- Human testis tissue; Human liver lysate
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General notes
The antibody solution should be gently mixed before use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/250 - 1/500. Predicted molecular weight: 10 kDa.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
| Notes |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/250 - 1/500. Predicted molecular weight: 10 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Cellular localization
Mitochondrion. -
Database links
- Entrez Gene: 64981 Human
- Omim: 611840 Human
- SwissProt: Q9BQ48 Human
- Unigene: 515242 Human
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Alternative names
- 39S ribosomal protein L34 mitochondrial antibody
- L34mt antibody
- Mitochondrial ribosomal protein L34 antibody
- MRP L34 antibody
Images
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Western blot - Anti-MRPL34 antibody (ab151055)All lanes : Anti-MRPL34 antibody (ab151055) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 10 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL34 antibody (ab151055)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling MRPL34 with ab151055 at 1/200 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-MRPL34 antibody (ab151055)Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, vesicles & actin filaments.
Protocols
Datasheets and documents
References (0)
ab151055 has not yet been referenced specifically in any publications.
