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    mrpl34-antibody-ab151055.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-MRPL34 antibody (ab151055)

  • Datasheet
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Western blot - Anti-MRPL34 antibody (ab151055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL34 antibody (ab151055)
  • Immunocytochemistry/ Immunofluorescence - Anti-MRPL34 antibody (ab151055)

Key features and details

  • Rabbit polyclonal to MRPL34
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-MRPL34 antibody
  • Description

    Rabbit polyclonal to MRPL34
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to amino acids 16-92 of Human MRPL34 (UniProt ID: Q9BQ48).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human testis tissue; Human liver lysate
  • General notes

    The antibody solution should be gently mixed before use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Mito. Translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/250 - 1/500. Predicted molecular weight: 10 kDa.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/250 - 1/500. Predicted molecular weight: 10 kDa.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Target

  • Cellular localization

    Mitochondrion.
  • Database links

    • Entrez Gene: 64981 Human
    • Omim: 611840 Human
    • SwissProt: Q9BQ48 Human
    • Unigene: 515242 Human
    • Alternative names

      • 39S ribosomal protein L34 mitochondrial antibody
      • L34mt antibody
      • Mitochondrial ribosomal protein L34 antibody
      • MRP L34 antibody

    Images

    • Western blot - Anti-MRPL34 antibody (ab151055)
      Western blot - Anti-MRPL34 antibody (ab151055)
      All lanes : Anti-MRPL34 antibody (ab151055) at 1/250 dilution

      Lane 1 : RT-4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human Plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Predicted band size: 10 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL34 antibody (ab151055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL34 antibody (ab151055)
      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling MRPL34 with ab151055 at 1/200 dilution.
    • Immunocytochemistry/ Immunofluorescence - Anti-MRPL34 antibody (ab151055)
      Immunocytochemistry/ Immunofluorescence - Anti-MRPL34 antibody (ab151055)
      Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, vesicles & actin filaments.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab151055? Please let us know so that we can cite the reference in this datasheet.

    ab151055 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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