Anti-MRPL41 antibody (ab121821)
Key features and details
- Rabbit polyclonal to MRPL41
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MRPL41 antibody -
Description
Rabbit polyclonal to MRPL41 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: KPYVSYLAPE SEETPLTAAQ LFSEAVAPAI EKDFKDGTFD PDNLEKYGFE PTQEGKLFQL YPRNFLR, corresponding to amino acids 71-137 of Human MRPL41.
-
Positive control
- Human rectum tissue; RT-4 and U-251 MG cell lysates; U-251MG cells
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab121821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. |
Target
-
Function
Component of the mitochondrial ribosome large subunit. Also involved in apoptosis and cell cycle. Enhances p53/TP53 stability, thereby contributing to p53/TP53-induced apoptosis in response to growth-inhibitory condition. Enhances p53/TP53 translocation to the mitochondria. Has the ability to arrest the cell cycle at the G1 phase, possibly by stabilizing the CDKN1A and CDKN1B (p27Kip1) proteins. -
Tissue specificity
Present in kidney, liver, thymus and testis, and at lower level in brain and spleen (at protein level). -
Sequence similarities
Belongs to the ribosomal protein L41 family. -
Cellular localization
Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 64975 Human
- Omim: 611846 Human
- SwissProt: Q8IXM3 Human
- Unigene: 44017 Human
-
Alternative names
- 39S ribosomal protein L27 homolog antibody
- 39S ribosomal protein L41 antibody
- 39S ribosomal protein L41 mitochondrial antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL41 antibody (ab121821)ab121821, at 1/300 dilution, staining MRPL41 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
-
Western blot - Anti-MRPL41 antibody (ab121821)All lanes : Anti-MRPL41 antibody (ab121821) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
Immunocytochemistry/ Immunofluorescence - Anti-MRPL41 antibody (ab121821)ab121821, at 4µg/ml, staining MRPL41 in U-251MG cells by Immunofluorescence. Antibody staining is shown in green.
Protocols
Datasheets and documents
References (0)
ab121821 has not yet been referenced specifically in any publications.
