Anti-MRPL41 antibody (ab121821)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MRPL41 antibody
See all MRPL41 primary antibodies -
Description
Rabbit polyclonal to MRPL41 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: KPYVSYLAPE SEETPLTAAQ LFSEAVAPAI EKDFKDGTFD PDNLEKYGFE PTQEGKLFQL YPRNFLR, corresponding to amino acids 71-137 of Human MRPL41.
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Positive control
- Human rectum tissue; RT-4 and U-251 MG cell lysates; U-251MG cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Component of the mitochondrial ribosome large subunit. Also involved in apoptosis and cell cycle. Enhances p53/TP53 stability, thereby contributing to p53/TP53-induced apoptosis in response to growth-inhibitory condition. Enhances p53/TP53 translocation to the mitochondria. Has the ability to arrest the cell cycle at the G1 phase, possibly by stabilizing the CDKN1A and CDKN1B (p27Kip1) proteins. -
Tissue specificity
Present in kidney, liver, thymus and testis, and at lower level in brain and spleen (at protein level). -
Sequence similarities
Belongs to the ribosomal protein L41 family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 64975 Human
- Omim: 611846 Human
- SwissProt: Q8IXM3 Human
- Unigene: 44017 Human
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Alternative names
- 39S ribosomal protein L27 homolog antibody
- 39S ribosomal protein L41 antibody
- 39S ribosomal protein L41 mitochondrial antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL41 antibody (ab121821)ab121821, at 1/300 dilution, staining MRPL41 in paraffin-embedded Human rectum tissue by Immunohistochemistry. -
Western blot - Anti-MRPL41 antibody (ab121821)All lanes : Anti-MRPL41 antibody (ab121821) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
Immunocytochemistry/ Immunofluorescence - Anti-MRPL41 antibody (ab121821)ab121821, at 4µg/ml, staining MRPL41 in U-251MG cells by Immunofluorescence. Antibody staining is shown in green.
Protocols
Datasheets and documents
References
ab121821 has not yet been referenced specifically in any publications.
