Overview

  • Product name
    Anti-MRPL42 antibody [3H6H2]
    See all MRPL42 primary antibodies
  • Description
    Mouse monoclonal [3H6H2] to MRPL42
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human MRPL42 aa 10-142. Expressed in E. Coli.
    Sequence:

    MSKRTILKHLFPVQNGALYCVCHKSTYSPLPDDYNCNVELALTSDGRTIV CYHPSVDIPYEHTKPIPRPDPVHNNEETHDQVLKTRLEEKVEHLEEGPMI EQLSKMFFTTKHRWYPHGRY HRCRKNLNPPKDR


    Database link: Q9Y6G3

  • Positive control
    • MRPL42 (aa: 10-142)-hIgGFc transfected HEK293 cell lysate; Human MRPL42 recombinant protein; HL7702, SMMC-7721, HEK293 , HeLa and Raji cell lysate; Human esophageal cancer and Human breast cancer tissues; HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab175368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 16 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 28S ribosomal protein S32 antibody
    • 39S ribosomal protein L31 antibody
    • 39S ribosomal protein L42 antibody
    • HSPC204 antibody
    • L31mt antibody
    • L42mt antibody
    • mitochondrial antibody
    • Mitochondrial ribosomal protein L42 antibody
    • Mitochondrial ribosomal protein S32 antibody
    • MRP L31 antibody
    • MRP L42 antibody
    • MRP S32 antibody
    • MRP-L31 antibody
    • MRP-L42 antibody
    • MRP-S32 antibody
    • MRPL31 antibody
    • Mrpl42 antibody
    • MRPS32 antibody
    • PTD007 antibody
    • RM42_HUMAN antibody
    • RPML31 antibody
    • S32mt antibody
    see all

Images

  • All lanes : Anti-MRPL42 antibody [3H6H2] (ab175368) at 1/500 dilution

    Lane 1 : HL7702 cell lysate.
    Lane 2 : SMMC-7721 cell lysate.
    Lane 3 : HEK293 cell lysate.
    Lane 4 : HeLa cell lysate.
    Lane 5 : Raji cell lysate.

    Predicted band size: 16 kDa

  • Anti-MRPL42 antibody [3H6H2] (ab175368) at 1/500 dilution + Human MRPL42 recombinant protein

    Predicted band size: 16 kDa



    Expected MW is 41.2 kDa

  • All lanes : Anti-MRPL42 antibody [3H6H2] (ab175368) at 1/500 dilution

    Lane 1 : Non transfected HEK293 cell lysate.
    Lane 2 : MRPL42 (aa: 10-142)-hIgGFc transfected HEK293 cell lysate.

    Predicted band size: 16 kDa



    Expected MW is 41.2 kDa

  • Flow cytometric analysis of HepG2 cells labeling MRPL42 using ab175368 at 1/200 dilution (green) and negative control (purple).

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues labeling MRPL42 using  ab175368 at 1/200 dilution, followed by DAB staining.

  • Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues labeling MRPL42 using ab175368 at 1/200 dilution, followed by DAB staining.

References

ab175368 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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