Anti-MRPL45 antibody (ab251748)
Key features and details
- Rabbit polyclonal to MRPL45
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MRPL45 antibody
See all MRPL45 primary antibodies -
Description
Rabbit polyclonal to MRPL45 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MRPL45 aa 50-200.
Database link: Q9BRJ2 -
Positive control
- IHC-P: Human liver, kidney, small intestine and prostate tissues. ICC/IF: U-2 OS cells. WB: RT4 and U-251 MG whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Sequence similarities
Belongs to the ribosomal protein L45 family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 84311 Human
- Omim: 611850 Human
- SwissProt: Q9BRJ2 Human
- Unigene: 537279 Human
- Unigene: 731941 Human
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Alternative names
- 39S ribosomal protein L45 antibody
- 39S ribosomal protein L45 mitochondrial antibody
- L45mt antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MRPL45 (green) using ab251748 at 4 µg/ml in ICC/IF.
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All lanes : Anti-MRPL45 antibody (ab251748) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 35 kDa -
Immunohistochemical analysis of human kidney tissue labeling MRPL45 with ab251748 at 1/1000 dilution.
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Immunohistochemical analysis of human liver tissue labeling MRPL45 with ab251748 at 1/1000 dilution.
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Immunohistochemical analysis of human prostate tissue labeling MRPL45 with ab251748 at 1/1000 dilution.
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Immunohistochemical analysis of human small intestine tissue labeling MRPL45 with ab251748 at 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab251748 has not yet been referenced specifically in any publications.