Overview

  • Product name

    Anti-MRPL46 antibody
  • Description

    Rabbit polyclonal to MRPL46
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to internal sequence amino acids of Human MRPL46 (NP_071446.2).

  • Positive control

    • Human lung (respiratory epithelium) tissue; HeLa cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab166873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Human lung (respiratory epithelium) tissue, labeling MRPL46 with ab166873 at 5 µg/ml.
  • All lanes : Anti-MRPL46 antibody (ab166873) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with synthesized peptide

    Predicted band size: 32 kDa

References

ab166873 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - other (Mouse whole brain homogenate, mitochondrial fracti)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gradient gel. Laemmli buffer with 10% β-Mercaptoethanol. Samples were denatured at 55C for 10 min. Immobilon-FL transfer membranes. Overnight transfer at +4C.)
Loading amount
20 µg
Specification
Mouse whole brain homogenate, mitochondrial fracti
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 10 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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