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    mrpl47-antibody-ab220005.pdf

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Anti-MRPL47 antibody (ab220005)

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Immunocytochemistry/ Immunofluorescence - Anti-MRPL47 antibody (ab220005)
  • Western blot - Anti-MRPL47 antibody (ab220005)

Key features and details

  • Rabbit polyclonal to MRPL47
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-MRPL47 antibody
  • Description

    Rabbit polyclonal to MRPL47
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MRPL47 aa 194-250.
    Sequence:

    YNRKRFFALPYVDHFLRLEREKRARIKARKENLERKKAKILLKKFPHLAE AQKSSLV


    Database link: Q9HD33
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • RT-4 and U-251 MG cell lysates; CACO-2 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Mito. Translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab220005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.25 - 2 µg/ml.
WB Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 29 kDa.

Target

  • Sequence similarities

    Belongs to the ribosomal protein L47em family.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession Q9HD33 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57129 Human
    • Omim: 611852 Human
    • SwissProt: Q9HD33 Human
    • Unigene: 283734 Human
    • Alternative names

      • 39S ribosomal protein L47 mitochondrial antibody
      • 39S ribosomal protein L47, mitochondrial antibody
      • CGI 204 antibody
      • L47mt antibody
      • MGC45403 antibody
      • Mitochondrial ribosomal protein L47 antibody
      • MRP L47 antibody
      • MRP-L47 antibody
      • MRPL 47 antibody
      • MRPL47 antibody
      • Nasopharyngeal carcinoma metastasis related 1 antibody
      • Nasopharyngeal carcinoma metastasis related protein 1 antibody
      • Nasopharyngeal carcinoma metastasis-related protein 1 antibody
      • NCM1 antibody
      • RM47_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-MRPL47 antibody (ab220005)
      Immunocytochemistry/ Immunofluorescence - Anti-MRPL47 antibody (ab220005)

      Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized CACO-2 cells labeling MRPL47 with ab220005 at 4 µg/mL (green).

    • Western blot - Anti-MRPL47 antibody (ab220005)
      Western blot - Anti-MRPL47 antibody (ab220005)
      All lanes : Anti-MRPL47 antibody (ab220005) at 1/250 dilution

      Lane 1 : RT-4 cell lysate
      Lane 2 : U-251 MG cell lysate

      Predicted band size: 29 kDa

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab220005? Please let us know so that we can cite the reference in this datasheet.

    ab220005 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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