Anti-MRPL52 antibody (ab121366)
Key features and details
- Rabbit polyclonal to MRPL52
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MRPL52 antibody -
Description
Rabbit polyclonal to MRPL52 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to amino acids 26-123 of Human MRPL52 (Q86TS9).
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Positive control
- RT-4 and U-251 MG, NIH-3T3 and NBT-II cell lysates, Human pancreas tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 14 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 14 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 122704 Human
- Entrez Gene: 68836 Mouse
- Omim: 611856 Human
- SwissProt: Q86TS9 Human
- SwissProt: Q9D0Y8 Mouse
- Unigene: 355935 Human
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Alternative names
- 39S ribosomal protein L52 antibody
- L52mt antibody
- mitochondrial antibody
see all
Images
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All lanes : Anti-MRPL52 antibody (ab121366) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma cell lysate
Lane 4 : Human Liver cell lysate
Lane 5 : Human Tonsil cell lysate
Predicted band size: 14 kDa -
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab121366 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-MRPL52 antibody (ab121366) at 1/250 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 14 kDa -
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelling MRPL52 with ab121366 antibody at a dilution of 1/50.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab121366 has been referenced in 2 publications.
- Li X et al. Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes. Front Cell Dev Biol 9:640667 (2021). PubMed: 33816490
- Li X et al. HIF-1-induced mitochondrial ribosome protein L52: a mechanism for breast cancer cellular adaptation and metastatic initiation in response to hypoxia. Theranostics 11:7337-7359 (2021). PubMed: 34158854