Product nameAnti-MRPP3 antibody [EPR14321-30]
See all MRPP3 primary antibodies
DescriptionRabbit monoclonal [EPR14321-30] to MRPP3
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human MRPP3 aa 1-150. The exact sequence is proprietary.
Database link: O15091
- K562, 293, HeLa and U87-MG cell lysates; HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185942 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 56-67 kDa (predicted molecular weight: 67 kDa).
Based on the sequence analysis by BLAST, ab185942 recognizes three isoforms with predicted MWs of 67KDa, 65KDa and 56KDa, respectively.
|IP||1/50 - 1/70.|
FunctionFunctions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends.
- Information by UniProt
- Kiaa0391 antibody
- KIAA0391 gene antibody
- Mitochondrial ribonuclease P protein 3 antibody
All lanes : Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/5000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 67 kDa
Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/1000 dilution + U87-MG cell lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 67 kDa
Immunofluorescent analysis of acetone-fixed HeLa cells labeling MRPP3 with ab185942 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Western blot analysis of MRPP3 in 293 cell lysate immunoprecipitated using ab185942 at 1/50 dilution (Lane 1). Lane 2: PBS instead of 293 lysate. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
ab185942 has not yet been referenced specifically in any publications.