Anti-MRPS10 antibody (ab111715)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MRPS10 antibody -
Description
Rabbit polyclonal to MRPS10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-178 of Human MRPS10 (AAH12560).
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Positive control
- NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; U373 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111715 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab111715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0) | |
| ICC/IF | 1/100 - 1/500. |
Target
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Relevance
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. [Entrez Gene] -
Cellular localization
Mitochondrion -
Database links
- Entrez Gene: 55173 Human
- Omim: 611976 Human
- SwissProt: P82664 Human
- Unigene: 380887 Human
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Alternative names
- Mitochondrial 28S ribosomal protein S10 antibody
- MRP S10 antibody
- PNAS 122 antibody
- S10mt antibody
Images
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Western blot - Anti-MRPS10 antibody (ab111715)Anti-MRPS10 antibody (ab111715) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 23 kDa
12% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-MRPS10 antibody (ab111715)ab111715 at 1/200 dilution staining MRPS10 in methanol-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS10 antibody (ab111715)ab111715 at 1/500 dilution staining MRPS10 in Formalin-fixed, paraffin-embedded U373 xenograft tissue by Immunohistochemistry.
Protocols
References
ab111715 has not yet been referenced specifically in any publications.
