Anti-MRPS10 antibody (ab111715)
Key features and details
- Rabbit polyclonal to MRPS10
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MRPS10 antibody -
Description
Rabbit polyclonal to MRPS10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-178 of Human MRPS10 (AAH12560).
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Positive control
- NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; U373 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111715 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab111715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0) | |
| ICC/IF | 1/100 - 1/500. |
Target
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Relevance
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. [Entrez Gene] -
Cellular localization
Mitochondrion -
Database links
- Entrez Gene: 55173 Human
- Omim: 611976 Human
- SwissProt: P82664 Human
- Unigene: 380887 Human
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Alternative names
- Mitochondrial 28S ribosomal protein S10 antibody
- MRP S10 antibody
- PNAS 122 antibody
- S10mt antibody
Images
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Western blot - Anti-MRPS10 antibody (ab111715)Anti-MRPS10 antibody (ab111715) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 23 kDa
12% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-MRPS10 antibody (ab111715)ab111715 at 1/200 dilution staining MRPS10 in methanol-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS10 antibody (ab111715)ab111715 at 1/500 dilution staining MRPS10 in Formalin-fixed, paraffin-embedded U373 xenograft tissue by Immunohistochemistry.
Protocols
References (0)
ab111715 has not yet been referenced specifically in any publications.
