Key features and details
- Rabbit polyclonal to MRPS15
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MRPS15 antibody
See all MRPS15 primary antibodies
DescriptionRabbit polyclonal to MRPS15
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MRPS15 aa 207-257. The exact sequence is proprietary.
Database link: P82914
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: MRPS15 IP in HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to MRPS15 immobilized on solid support.
Our Abpromise guarantee covers the use of ab242120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the ribosomal protein S15P family.
- Information by UniProt
- 28S ribosomal protein S15 antibody
- 28S ribosomal protein S15, mitochondrial [Precursor] antibody
- DC37 antibody
All lanes : Anti-MRPS15 antibody (ab242120) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
MRPS15 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242120 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab242120 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242120 has not yet been referenced specifically in any publications.