Anti-MRPS15 antibody (ab242120)
Key features and details
- Rabbit polyclonal to MRPS15
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MRPS15 antibody
See all MRPS15 primary antibodies -
Description
Rabbit polyclonal to MRPS15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MRPS15 aa 207-257. The exact sequence is proprietary.
Database link: P82914 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: MRPS15 IP in HEK-293T whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to MRPS15 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/25000. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Sequence similarities
Belongs to the ribosomal protein S15P family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 64960 Human
- Omim: 611979 Human
- SwissProt: P82914 Human
- Unigene: 352839 Human
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Alternative names
- 28S ribosomal protein S15 antibody
- 28S ribosomal protein S15, mitochondrial [Precursor] antibody
- DC37 antibody
see all
Images
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Western blot - Anti-MRPS15 antibody (ab242120)All lanes : Anti-MRPS15 antibody (ab242120) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-MRPS15 antibody (ab242120)MRPS15 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242120 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.Lane 1: ab242120 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242120 has not yet been referenced specifically in any publications.
