Product nameAnti-MRPS15 antibody
See all MRPS15 primary antibodies
DescriptionRabbit polyclonal to MRPS15
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MRPS15 aa 207-257. The exact sequence is proprietary.
Database link: P82914
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: MRPS15 IP in HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to MRPS15 immobilized on solid support.
Our Abpromise guarantee covers the use of ab242120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the ribosomal protein S15P family.
- Information by UniProt
- 28S ribosomal protein S15 antibody
- 28S ribosomal protein S15, mitochondrial [Precursor] antibody
- DC37 antibody
All lanes : Anti-MRPS15 antibody (ab242120) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
MRPS15 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242120 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab242120 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
ab242120 has not yet been referenced specifically in any publications.