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    mrps15-antibody-ab242120.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-MRPS15 antibody (ab242120)

  • Datasheet
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Abpromise

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Western blot - Anti-MRPS15 antibody (ab242120)
  • Immunoprecipitation - Anti-MRPS15 antibody (ab242120)

Key features and details

  • Rabbit polyclonal to MRPS15
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-MRPS15 antibody
    See all MRPS15 primary antibodies
  • Description

    Rabbit polyclonal to MRPS15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MRPS15 aa 207-257. The exact sequence is proprietary.
    Database link: P82914

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: MRPS15 IP in HEK-293T whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to MRPS15 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Mito. Translation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab242120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the ribosomal protein S15P family.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession P82914 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64960 Human
    • Omim: 611979 Human
    • SwissProt: P82914 Human
    • Unigene: 352839 Human
    • Alternative names

      • 28S ribosomal protein S15 antibody
      • 28S ribosomal protein S15, mitochondrial [Precursor] antibody
      • DC37 antibody
      • FLJ11564 antibody
      • mitochondrial antibody
      • mitochondrial ribosomal protein S15 antibody
      • MPR S15 antibody
      • MRP-S15 antibody
      • MRPS15 antibody
      • RPMS15 antibody
      • RT15_HUMAN antibody
      • S15mt antibody
      see all

    Images

    • Western blot - Anti-MRPS15 antibody (ab242120)
      Western blot - Anti-MRPS15 antibody (ab242120)
      All lanes : Anti-MRPS15 antibody (ab242120) at 0.04 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Exposure time: 30 seconds


      Prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-MRPS15 antibody (ab242120)
      Immunoprecipitation - Anti-MRPS15 antibody (ab242120)

      MRPS15 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
      ab242120 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.

      Lane 1: ab242120 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG.

      Chemiluminescence detection: 30 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab242120? Please let us know so that we can cite the reference in this datasheet.

    ab242120 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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