Anti-MRPS15 antibody (ab242120)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MRPS15 antibody
See all MRPS15 primary antibodies -
Description
Rabbit polyclonal to MRPS15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MRPS15 aa 207-257. The exact sequence is proprietary.
Database link: P82914 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: MRPS15 IP in HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to MRPS15 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/25000. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Sequence similarities
Belongs to the ribosomal protein S15P family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 64960 Human
- Omim: 611979 Human
- SwissProt: P82914 Human
- Unigene: 352839 Human
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Alternative names
- 28S ribosomal protein S15 antibody
- 28S ribosomal protein S15, mitochondrial [Precursor] antibody
- DC37 antibody
see all
Images
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Western blot - Anti-MRPS15 antibody (ab242120)All lanes : Anti-MRPS15 antibody (ab242120) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-MRPS15 antibody (ab242120)MRPS15 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242120 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.Lane 1: ab242120 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
Datasheets and documents
References
ab242120 has not yet been referenced specifically in any publications.
