Overview

  • Product name

    Anti-MRPS15 antibody [EPR9361]
    See all MRPS15 primary antibodies
  • Description

    Rabbit monoclonal [EPR9361] to MRPS15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MRPS15 aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • U-87 MG, Raji and HeLa cell lysates; Human breast carcinoma tissue; HeLa and U-87 MG cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Sequence similarities

      Belongs to the ribosomal protein S15P family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • 28S ribosomal protein S15 antibody
      • 28S ribosomal protein S15, mitochondrial [Precursor] antibody
      • DC37 antibody
      • FLJ11564 antibody
      • mitochondrial antibody
      • mitochondrial ribosomal protein S15 antibody
      • MPR S15 antibody
      • MRP-S15 antibody
      • MRPS15 antibody
      • RPMS15 antibody
      • RT15_HUMAN antibody
      • S15mt antibody
      see all

    Images

    • All lanes : Anti-MRPS15 antibody [EPR9361] (ab137070) at 1/1000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 30 kDa

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MRPS15 with ab137070 at 1/100 dilution.
    • Immunofluorescent analysis of HeLa cells labelling MRPS15 with ab137070 at 1/250 dilution.
    • Immunofluorescent analysis of U-87 MG cells labelling MRPS15 with ab137070 at 1/250 dilution.

    References

    This product has been referenced in:

    • Lee YK  et al. Lactate-mediated mitoribosomal defects impair mitochondrial oxidative phosphorylation and promote hepatoma cell invasiveness. J Biol Chem 292:20208-20217 (2017). Read more (PubMed: 28978646) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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