Recombinant Anti-MRPS15 antibody [EPR9361] (ab137070)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9361] to MRPS15
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MRPS15 antibody [EPR9361]
See all MRPS15 primary antibodies -
Description
Rabbit monoclonal [EPR9361] to MRPS15 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MRPS15 aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- U-87 MG, Raji and HeLa cell lysates; Human breast carcinoma tissue; HeLa and U-87 MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9361 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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Notes |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
Target
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Sequence similarities
Belongs to the ribosomal protein S15P family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 64960 Human
- Omim: 611979 Human
- SwissProt: P82914 Human
- Unigene: 352839 Human
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Alternative names
- 28S ribosomal protein S15 antibody
- 28S ribosomal protein S15, mitochondrial [Precursor] antibody
- DC37 antibody
see all
Images
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All lanes : Anti-MRPS15 antibody [EPR9361] (ab137070) at 1/1000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MRPS15 with ab137070 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labelling MRPS15 with ab137070 at 1/250 dilution.
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Immunofluorescent analysis of U-87 MG cells labelling MRPS15 with ab137070 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137070 has been referenced in 4 publications.
- Min S et al. Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection. Cells 11:N/A (2022). PubMed: 35805162
- Chen J et al. Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer. Cancer Cell Int 21:136 (2021). PubMed: 33632224
- Min S et al. MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness. Cell Death Dis 12:1076 (2021). WB ; Human . PubMed: 34772924
- Lee YK et al. Lactate-mediated mitoribosomal defects impair mitochondrial oxidative phosphorylation and promote hepatoma cell invasiveness. J Biol Chem 292:20208-20217 (2017). PubMed: 28978646