Recombinant Anti-MRPS17 antibody [EPR12583] (ab175207)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12583] to MRPS17
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MRPS17 antibody [EPR12583]
See all MRPS17 primary antibodies -
Description
Rabbit monoclonal [EPR12583] to MRPS17 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human MRPS17. The exact sequence is proprietary.
Database link: Q9Y2R5 -
Positive control
- HeLa, HepG2 and U937 cell lysate; Human kidney and liver tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12583 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 15 kDa.
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Predicted molecular weight: 15 kDa. |
ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
Target
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Sequence similarities
Belongs to the ribosomal protein S17P family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 51373 Human
- Omim: 611980 Human
- SwissProt: Q9Y2R5 Human
- Unigene: 44298 Human
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Alternative names
- 1810006P02Rik antibody
- 28S ribosomal protein S17 antibody
- 28S ribosomal protein S17 mitochondrial antibody
see all
Images
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All lanes : Anti-MRPS17 antibody [EPR12583] (ab175207) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa -
Immunofluorescent staining of HeLa cells labeling MRPS17 with ab175207 at 1/100 dilution (green). DAPI nuclear staining (blue).
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling MRPS17 with ab175207 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human liver tissue labeling MRPS17 with ab175207 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling MRPS17 with ab175207 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175207 has not yet been referenced specifically in any publications.