Anti-MRPS21 antibody (ab74101)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MRPS21 antibody
See all MRPS21 primary antibodies -
Description
Rabbit polyclonal to MRPS21 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
synthetic peptide derived from internal of human MRPS21.
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Positive control
- human pancreas tissue, extracts from COS cells, MCF-7 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab74101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 11 kDa. | |
| ELISA | 1/40000. | |
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | 1/500 - 1/1000. |
Target
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Relevance
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein. [provided by RefSeq] -
Cellular localization
Mitochondrial small ribosomal subunit -
Database links
- Entrez Gene: 54460 Human
- Entrez Gene: 66292 Mouse
- Entrez Gene: 689432 Rat
- Omim: 611984 Human
- SwissProt: P82921 Human
- SwissProt: P58059 Mouse
- SwissProt: B2RYT0 Rat
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Alternative names
- MDS016 antibody
- mitochondrial 28S ribosomal protein S21 antibody
- Mitochondrial ribosomal protein S21 antibody
see all
Images
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Western blot - Anti-MRPS21 antibody (ab74101)All lanes : Anti-MRPS21 antibody (ab74101) at 1/500 dilution
Lane 1 : extracts from COS cells
Lane 2 : extracts from COS cells with immunizing peptide at 5 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS21 antibody (ab74101)Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using 1/50 ab74101 with (right panel) or without (left panel) preincubation with immunizing peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-MRPS21 antibody (ab74101)Immunofluorescence analysis of MCF-7 cells, using 1/500 ab74101 with (right panel) or without (left panel) preincubation with immunizing peptide.
Protocols
Datasheets and documents
References
ab74101 has not yet been referenced specifically in any publications.
