Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14396] to MRPS26
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-MRPS26 antibody [EPR14396]
See all MRPS26 primary antibodies
DescriptionRabbit monoclonal [EPR14396] to MRPS26
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human MRPS26 aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BYN8
- HeLa, HepG2 and Raji cell lysates; HeLa and HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
RelevanceMammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. This gene lies adjacent to and downstream of the gonadotropin-releasing hormone precursor gene.
Cellular localizationMitochondrion: mitochondrial small ribosomal subunit
- 28S ribosomal protein S13 mitochondrial antibody
- 28S ribosomal protein S26 mitochondrial antibody
- C20orf193 antibody
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling MRPS26 with ab181863 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
All lanes : Anti-MRPS26 antibody [EPR14396] (ab181863) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 24 kDa
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling MRPS26 with ab181863 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
ab181863 has not yet been referenced specifically in any publications.