Key features and details
- Rabbit polyclonal to MRPS27
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MRPS27 antibody
DescriptionRabbit polyclonal to MRPS27
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 1-153 of Human MRPS27 (UniProt: Q92552).
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
Sequence similaritiesContains 2 PPR (pentatricopeptide) repeats.
- Information by UniProt
- 28S ribosomal protein S27 antibody
- 28S ribosomal protein S27, mitochondrial antibody
- KIAA0264 antibody
Anti-MRPS27 antibody (ab153940) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 48 kDa
12% SDS PAGE
Immunohistochemistry analysis of paraffin-embedded Human Colon carcinoma tissue labeling MRPS27 with ab153940 at 1/250 dilution.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling MRPS27 with ab153940 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
ab153940 has not yet been referenced specifically in any publications.