Overview

  • Product name

    Anti-MS2 antibody [EPR22688-44]
    See all MS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR22688-44] to MS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt,IP or WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment aa 1-450. The exact sequence is proprietary.
    Database link: P78325

  • Positive control

    • IHC-P: Human colon, Human breast cancer, Mouse colon and Rat colon tissues. ICC/IF: 293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab255608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.

 

 

ICC/IF 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IP or WB.
  • Target

    • Function

      Possible involvement in extravasation of leukocytes.
    • Tissue specificity

      Expressed on neutrophils and monocytes.
    • Sequence similarities

      Contains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • A Disintegrin And Metalloproteinase domain 8 antibody
      • ADAM 8 antibody
      • ADAM 8 precursor antibody
      • ADAM metallopeptidase domain 8 antibody
      • Adam8 antibody
      • ADAM8 protein antibody
      • ADAM8_HUMAN antibody
      • CD 156 antibody
      • CD156 antibody
      • CD156a antibody
      • CD156a antigen antibody
      • Cell surface antigen MS2 antibody
      • Disintegrin and metalloproteinase domain-containing protein 8 antibody
      • Human leukocyte differentiation antigen antibody
      • Macrophage cysteine rich glycoprotein antibody
      • MGC134985 antibody
      • MS 2 antibody
      • MS2 antibody
      see all

    Images

    • Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling ADAM8 with ab255608 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged ADAM8 expression vector. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling ADAM8 with ab255608 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in immune cells of rat colon (PMID: 17270707) Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling ADAM8 with ab255608 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in immune cells of mouse colon (PMID: 17270707) Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ADAM8 with ab255608 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID: 24375628, PMID:30635388) Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ADAM8 with ab255608 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in immune cells of human colon (PMID: 17270707) The population of positively stained cells depends on cases. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    References

    ab255608 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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