Recombinant Anti-MS2 antibody [EPR22688-44] (ab255608)
![Immunocytochemistry/ Immunofluorescence - Anti-MS2 antibody [EPR22688-44] (ab255608)](https://www.abcam.com/ps/products/255/ab255608/Images/ab255608-5-anti-adam8-antibody-epr22688-44-immunocytochemistry-293t-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MS2 antibody [EPR22688-44] (ab255608)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22688-44] to MS2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MS2 antibody [EPR22688-44]
See all MS2 primary antibodies -
Description
Rabbit monoclonal [EPR22688-44] to MS2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt,IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment aa 1-450. The exact sequence is proprietary.
Database link: P78325 -
Positive control
- IHC-P: Human colon, Human breast cancer, Mouse colon and Rat colon tissues. ICC/IF: 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.5% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22688-44 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab255608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/2000.
|
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| ICC/IF | 1/100. |
Target
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Function
Possible involvement in extravasation of leukocytes. -
Tissue specificity
Expressed on neutrophils and monocytes. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 101 Human
- Entrez Gene: 11501 Mouse
- Entrez Gene: 499285 Rat
- Omim: 602267 Human
- SwissProt: P78325 Human
- SwissProt: Q05910 Mouse
- Unigene: 501574 Human
- Unigene: 15969 Mouse
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Alternative names
- A Disintegrin And Metalloproteinase domain 8 antibody
- ADAM 8 antibody
- ADAM 8 precursor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MS2 antibody [EPR22688-44] (ab255608)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling ADAM8 with ab255608 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged ADAM8 expression vector. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody [EPR22688-44] (ab255608)](https://www.abcam.com/ps/products/255/ab255608/Images/ab255608-4-anti-adam8-antibody-epr22688-44-immunohistochemistry-colon-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody [EPR22688-44] (ab255608)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling ADAM8 with ab255608 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in immune cells of rat colon (PMID: 17270707) Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody [EPR22688-44] (ab255608)](https://www.abcam.com/ps/products/255/ab255608/Images/ab255608-3-anti-adam8-antibody-epr22688-44-immunohistochemistry-colon-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody [EPR22688-44] (ab255608)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling ADAM8 with ab255608 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in immune cells of mouse colon (PMID: 17270707) Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody [EPR22688-44] (ab255608)](https://www.abcam.com/ps/products/255/ab255608/Images/ab255608-2-anti-adam8-antibody-epr22688-44-immunohistochemistry-breast-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody [EPR22688-44] (ab255608)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ADAM8 with ab255608 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID: 24375628, PMID:30635388) Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody [EPR22688-44] (ab255608)](https://www.abcam.com/ps/products/255/ab255608/Images/ab255608-1-anti-adam8-antibody-epr22688-44-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody [EPR22688-44] (ab255608)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ADAM8 with ab255608 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in immune cells of human colon (PMID: 17270707) The population of positively stained cells depends on cases. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-MS2 antibody [EPR22688-44] (ab255608)](https://www.abcam.com/ps/products/255/ab255608/Images/ab255608-6-benefits-of-recombinant-antibodies.png)
Anti-MS2 antibody [EPR22688-44] (ab255608)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab255608 has not yet been referenced specifically in any publications.
