Anti-MS4A4A antibody (ab67134)

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Overview

  • Product name

    Anti-MS4A4A antibody

  • Description

    Mouse polyclonal to MS4A4A

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length MS4A4A protein (Human)

  • Positive control

    • Human spleen tissue lysate and MS4A4A transfected 293T cell lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab67134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects bands of approximately 20 and 30 kDa in separate blots (predicted molecular weight: 25 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      MS4A4A is a member of the membrane spanning 4A gene family. This nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and non lymphoid tissues. MS4A4A may be involved in signal transduction as a component of a multimeric receptor complex. There are two named isoforms.

    • Cellular localization

      Cell Membrane; Multi pass membrane protein.

    • Database links

      • Alternative names

        • 4SPAN1 antibody
        • CD20 antigen like 1 antibody
        • CD20L1 antibody
        • MS4A4 antibody

      Images

      • Western blot - Anti-MS4A4A antibody (ab67134)
        Western blot - Anti-MS4A4A antibody (ab67134)
        Anti-MS4A4A antibody (ab67134) at 1/500 dilution + human spleen tissue lysate at 25 µg/ml

        Secondary
        Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

        Predicted band size: 25 kDa
        Observed band size: 30 kDa
        why is the actual band size different from the predicted?

      • Western blot - Anti-MS4A4A antibody (ab67134)
        Western blot - Anti-MS4A4A antibody (ab67134)
        All lanes : Anti-MS4A4A antibody (ab67134) at 1/500 dilution

        Lane 1 : MS4A4A transfected 293T cell lysate
        Lane 2 : Non-transfected 293T cell lysate

        Lysates/proteins at 25 µg/ml per lane.

        Secondary
        All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

        Predicted band size: 25 kDa
        Observed band size: 20 kDa why is the actual band size different from the predicted?

      References

      ab67134 has not yet been referenced specifically in any publications.

      Publishing research using ab67134? Please let us know so that we can cite the reference in this datasheet.

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