Anti-MSC antibody (ab221097)

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Overview

  • Product name

    Anti-MSC antibody
    See all MSC primary antibodies

  • Description

    Rabbit polyclonal to MSC

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human MSC aa 3-45.
    Sequence:

    TGSVSDPEEMELRGLQREYPVPASKRPPLRGVERSYASPSDNS


    Database link: O60682

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: RT-4 cell lysate. ICC/IF: SK-MEL-30 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100
WB 1/100 - 1/250. Predicted molecular weight: 22 kDa.

Target

  • Function

    Transcription repressor capable of inhibiting the transactivation capability of TCF3/E47. May play a role in regulating antigen-dependent B-cell differentiation.

  • Tissue specificity

    Expressed in lymphoid tissues, B-cell lines and activated B-cells.

  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • ABF-1 antibody
      • ABF1 antibody
      • Activated B cell factor 1 antibody
      • Activated B-cell factor 1 antibody
      • bHLHa22 antibody
      • Class A basic helix-loop-helix protein 22 antibody
      • Msc antibody
      • MUSC_HUMAN antibody
      • Musculin antibody
      • MYOR antibody
      see all

    Images

    • Western blot - Anti-MSC antibody (ab221097)
      Western blot - Anti-MSC antibody (ab221097)
      Anti-MSC antibody (ab221097) at 1/100 dilution + RT-4 cell lysate

      Predicted band size: 22 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MSC antibody (ab221097)
      Immunocytochemistry/ Immunofluorescence - Anti-MSC antibody (ab221097)

      Immunofluorescent analysis of paraformaldehyde-fixed, Triton X-100 permeabilized SK-MEL-30 cells labeling MSC with ab221097 at 4 μg/mL dilution (green). 

    References

    ab221097 has not yet been referenced specifically in any publications.

    Publishing research using ab221097? Please let us know so that we can cite the reference in this datasheet.

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