The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
Involvement in disease
Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Belongs to the DNA mismatch repair mutS family. MSH3 subfamily.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Keogh N et al. MutSß abundance and Msh3 ATP hydrolysis activity are important drivers of CTG•CAG repeat expansions. Nucleic Acids Res45:10068-10078 (2017).
Read more (PubMed: 28973443) »
Zhao J et al. Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks. Nucleic Acids Res37:4420-9 (2009).
Read more (PubMed: 19468048) »