- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-MSH3 antibody
See all MSH3 primary antibodies -
DescriptionRabbit polyclonal to MSH3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide derived from an internal region of human MSH3.
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Positive control
- Extracts from HUVEC cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab69619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa). | |
| ELISA | 1/20000. |
Target
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FunctionComponent of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
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Involvement in diseaseDefects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
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Sequence similaritiesBelongs to the DNA mismatch repair mutS family. MSH3 subfamily.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 4437 Human
- Omim: 600887 Human
- SwissProt: P20585 Human
- Unigene: 280987 Human
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Alternative names
- Divergent upstream protein antibody
- DNA mismatch repair protein antibody
- DNA mismatch repair protein Msh 3 antibody
see all
Images
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Western blot - Anti-MSH3 antibody (ab69619)All lanes : Anti-MSH3 antibody (ab69619) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Protocols
References
This product has been referenced in:
- Keogh N et al. MutSß abundance and Msh3 ATP hydrolysis activity are important drivers of CTG•CAG repeat expansions. Nucleic Acids Res 45:10068-10078 (2017). Read more (PubMed: 28973443) »
- Zhao J et al. Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks. Nucleic Acids Res 37:4420-9 (2009). WB ; Human . Read more (PubMed: 19468048) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"