Key features and details
- Rabbit polyclonal to MSH3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MSH3 antibody
See all MSH3 primary antibodies
DescriptionRabbit polyclonal to MSH3
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MSH3 aa 350-450 (internal sequence). The exact sequence is proprietary.
Database link: P20585
- Human rectal carcinoma tissue
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunogen affinity purified immunoglobulin from rabbit serum.
Our Abpromise guarantee covers the use of ab93663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionComponent of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
Involvement in diseaseDefects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Sequence similaritiesBelongs to the DNA mismatch repair mutS family. MSH3 subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Divergent upstream protein antibody
- DNA mismatch repair protein antibody
- DNA mismatch repair protein Msh 3 antibody
ab93663, at a 1/100 dilution, staining MSH3 in formalin fixed, paraffin embedded Human rectal carcinoma tissue by Immunohistochemistry.
ICC/IF image of ab93663 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93663, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab93663 has not yet been referenced specifically in any publications.