Key features and details
- Rabbit polyclonal to MSH3 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MSH3 antibody - C-terminal
See all MSH3 primary antibodies
DescriptionRabbit polyclonal to MSH3 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Recombinant protein fragment, corresponding to a region within C terminal amino acids 916-1137 of Human MSH3 (UniProt ID: P20585).
- A431 whole cell lysate (ab7909) can be used as a positive control in WB. A549 cell lysate; HeLa cells; Human colon carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 127 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionComponent of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
Involvement in diseaseDefects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Sequence similaritiesBelongs to the DNA mismatch repair mutS family. MSH3 subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Divergent upstream protein antibody
- DNA mismatch repair protein antibody
- DNA mismatch repair protein Msh 3 antibody
Anti-MSH3 antibody - C-terminal (ab154521) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 127 kDa
5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling MSH3 using ab154521 at a 1/500 dilution. image in the lower panel was costained with Hoechst 33342.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, labeling MSH3 using ab154521 at a 1/500 dilution.
ab154521 has not yet been referenced specifically in any publications.