Overview

  • Product name

    Anti-MSH3 antibody [EPR4334(2)] - BSA and Azide free
    See all MSH3 primary antibodies
  • Description

    Rabbit monoclonal [EPR4334(2)] to MSH3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MSH3 aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: P20585

  • Positive control

    • Human colon adenocarcinoma and tonsil tissues; SW480 cells.
  • General notes

    Ab226079 is the carrier-free version of ab111107. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226079 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4334(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For antigen retrieval, heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
    • Involvement in disease

      Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    • Sequence similarities

      Belongs to the DNA mismatch repair mutS family. MSH3 subfamily.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links

    • Alternative names

      • Divergent upstream protein antibody
      • DNA mismatch repair protein antibody
      • DNA mismatch repair protein Msh 3 antibody
      • DNA mismatch repair protein MSH3 antibody
      • DUC 1 antibody
      • DUC1 antibody
      • DUG antibody
      • DUP antibody
      • hMSH3 antibody
      • MGC163306 antibody
      • MGC163308 antibody
      • Mismatch repair protein 1 antibody
      • MRP 1 antibody
      • MRP1 antibody
      • MSH 3 antibody
      • MSH3 antibody
      • MSH3_HUMAN antibody
      • MutS homolog 3 (E. coli) antibody
      • MutS homolog 3 antibody
      see all

    References

    ab226079 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226079.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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