Overview

  • Product name

  • Description

    Rabbit polyclonal to MSH4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    A synthetic peptide corresponding to C terminal residues of Mouse MSH4

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19247432
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis.
  • Tissue specificity

    Testis and ovary specific.
  • Sequence similarities

    Belongs to the DNA mismatch repair MutS family.
  • Information by UniProt
  • Database links

  • Alternative names

    • hMSH4 antibody
    • MSH 4 antibody
    • MSH4 antibody
    • MSH4_HUMAN antibody
    • MutS homolog 4 (E. Coli) antibody
    • MutS homolog 4 antibody
    • MutS protein homolog 4 antibody
    see all

Images

  • ab58666 staining MSH4 in mouse spermatocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 1% paraformaldehyde and 0.15% Triton X-100. An Alexa flour conjugated anti rabbit IgG was used as secondary. Green colour staining was observed with ab58666.

References

This product has been referenced in:

See all 14 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (spermatocytes)
Permeabilization
No
Specification
spermatocytes
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 01 2016

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