Overview

  • Product name

  • Description

    Rabbit polyclonal to MSH4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide from a region within N terminal amino acids 108-157 (SARDTNYPQT LKTPLSTGNP QRSGYKSWTP QVGYSASSSS AISAHSPSVI) of Human MSH4 (NP_002431).

  • Positive control

    • PANC-1 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-MSH4 antibody (ab95096) at 1 µg/ml (in 5% skim milk / PBS buffer) + PANC-1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 105 kDa



    Gel concentration: 6-18%

References

This product has been referenced in:

  • Sun X  et al. FancJ (Brip1) loss-of-function allele results in spermatogonial cell depletion during embryogenesis and altered processing of crossover sites during meiotic prophase I in mice. Chromosoma 125:237-52 (2016). Read more (PubMed: 26490168) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spermatocytes)
Permeabilization
No
Specification
Spermatocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 02 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up