Overview

  • Product name

    Anti-MSH5 antibody
  • Description

    Goat polyclonal to MSH5
  • Host species

    Goat
  • Specificity

    ab129268 is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey
  • Immunogen

    Synthetic peptide:

    GDLHCEIRDQET

    , corresponding to internal sequence amino acids 491-502 of Human MSH5 (NP_079535.3 ; NP_751897.1; NP_002432.1).

  • Positive control

    • Human testis and thymus tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Preliminary experiments gave an approx 38kD band in Human Heart, Tonsil and Thymus lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kD according to the smallest reported isoform NP_002432.1. The 38kD band was successfully blocked by incubation with the immunizing peptide.
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab129268, at 3.75 µg/ml, staining MSH5 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.
  • ab129268, at 3.75 µg/ml, staining MSH5 in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.

References

ab129268 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Nuclear surface spreads from testis cell suspensio)
Permeabilization
No
Specification
Nuclear surface spreads from testis cell suspensio
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 08 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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