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    msh6-antibody-ab137457.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Mismatch Repair
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Anti-MSH6 antibody (ab137457)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Western blot - Anti-MSH6 antibody (ab137457)
  • Western blot - Anti-MSH6 antibody (ab137457)
  • Western blot - Anti-MSH6 antibody (ab137457)
  • Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody (ab137457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody (ab137457)

Key features and details

  • Rabbit polyclonal to MSH6
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human MSH6 protein (ab152443)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MSH6 antibody
    See all MSH6 primary antibodies
  • Description

    Rabbit polyclonal to MSH6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 825-1110 of Human MSH6 (NP_000170).

  • Positive control

    • HepG2, Neuro2A and GL261 whole cell lysates; HeLa cells; NCIN87 xenograft tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Mismatch Repair

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human MSH6 protein (ab152443)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 153 kDa.
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 153 kDa.
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.
  • Involvement in disease

    Defects in MSH6 are the cause of hereditary non-polyposis colorectal cancer type 5 (HNPCC5) [MIM:600678]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. MSH6 mutations appear to be associated with atypical HNPCC and in particular with development of endometrial carcinoma or atypical endometrial hyperplasia, the presumed precursor of endometrial cancer. Defects in MSH6 are also found in familial colorectal cancers (suspected or incomplete HNPCC) that do not fulfill the Amsterdam criteria for HNPCC.
    Defects in MSH6 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
  • Sequence similarities

    Belongs to the DNA mismatch repair mutS family.
    Contains 1 PWWP domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P52701 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2956 Human
    • Entrez Gene: 17688 Mouse
    • Omim: 600678 Human
    • SwissProt: P52701 Human
    • SwissProt: P54276 Mouse
    • Unigene: 445052 Human
    • Unigene: 18210 Mouse
    • Alternative names

      • DNA mismatch repair protein Msh6 antibody
      • G/T mismatch binding protein antibody
      • G/T mismatch-binding protein antibody
      • GTBP antibody
      • GTMBP antibody
      • hMSH6 antibody
      • HNPCC 5 antibody
      • HNPCC5 antibody
      • HSAP antibody
      • MSH 6 antibody
      • MSH6 antibody
      • MSH6_HUMAN antibody
      • mutS (E. coli) homolog 6 antibody
      • MutS alpha 160 kDa subunit antibody
      • MutS homolog 6 (E. coli) antibody
      • mutS homolog 6 antibody
      • MutS-alpha 160 kDa subunit antibody
      • p160 antibody
      • Sperm associated protein antibody
      see all

    Images

    • Western blot - Anti-MSH6 antibody (ab137457)
      Western blot - Anti-MSH6 antibody (ab137457)
      All lanes : Anti-MSH6 antibody (ab137457) at 1/1000 dilution

      Lane 1 : WT HeLa cell extract
      Lane 2 : MSH6 Knockout HeLa cell extracts

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG

      Predicted band size: 153 kDa

    • Western blot - Anti-MSH6 antibody (ab137457)
      Western blot - Anti-MSH6 antibody (ab137457)
      Anti-MSH6 antibody (ab137457) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

      Predicted band size: 153 kDa



      5% SDS PAGE
    • Western blot - Anti-MSH6 antibody (ab137457)
      Western blot - Anti-MSH6 antibody (ab137457)
      All lanes : Anti-MSH6 antibody (ab137457) at 1/1000 dilution

      Lane 1 : Neuro2A whole cell lysate
      Lane 2 : GL261 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 153 kDa



      5% SDS-PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody (ab137457)
      Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody (ab137457)
      Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling MSH6 using ab137457 at 1/500 dilution. Right panel merged with DNA probe.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody (ab137457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody (ab137457)
      Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue labelling MSH6 using ab137457 at 1/100 dilution.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab137457? Please let us know so that we can cite the reference in this datasheet.

    ab137457 has been referenced in 2 publications.

    • Liu S  et al. Okazaki fragment maturation involves a-segment error editing by the mammalian FEN1/MutSa functional complex. EMBO J 34:1829-43 (2015). PubMed: 25921062
    • Cen L  et al. Efficacy of protracted temozolomide dosing is limited in MGMT unmethylated GBM xenograft models. Neuro Oncol 15:735-46 (2013). PubMed: 23479134

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    One of our customer want to know whichantibody will give best result
    for thediagnosis of Annexin A1 as IHC marker of Hairy cell Leukaemia
    on Bone marrow Biopsy. And they also want to know which antibody
    would bebest for diagnostic purposes using human formalin fixed paraffin
    embedded tissue for IDH1, MDM2,MSI (MHS6, MSH2, MLH1, PMS2),
    Napsin, H-caldesmon and P16.They use the Leica Bondmax to perform the
    IHCs, sothe secondary antibody detection kit is a closed system allowingthem
    to use Novacastra Bond Polymer Refine Detection kit (catalogue no DS9800).
    they prefer pre-diluted ready use antibodies if available. So please suggest the
    suitable product for them.

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 08 2012

    Answer

    Thank you for contacting us.

    I am assuming the species customer of tissue sections is human - antibodeis are asfollows;

    The best anti annexin a1 antibody is ab47661;https://www.abcam.com/Annexin-A1-antibody-ab47661.html

    anti IDH1 ab135659; https://www.abcam.com/Isocitrate-dehydrogenase-antibody-ab135659.html

    anti MDM2, ab38618; https://www.abcam.com/MDM2-antibody-ab38618.html

    anti PMS2, ab111253; https://www.abcam.com/PMS2-antibody-ab111253.html

    ab9144; https://www.abcam.com/MLH1-antibody-ab9144.html

    MSH2, ab16833; https://www.abcam.com/MSH2-antibody-ab16833.html

    MSH6, ab137457; https://www.abcam.com/MSH6-antibody-ab137457.html

    anti Napsin, ab73098; https://www.abcam.com/NAPSIN-A-antibody-KCG1-1-prediluted-ab73098.html

    Caldesmon, ab68878; https://www.abcam.com/Caldesmon-antibody-ab68878.html

    p16????? ab118459; https://www.abcam.com/p16-ARC-antibody-ab118459.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 08 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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