Overview

  • Product name

  • Description

    Rabbit polyclonal to MSH6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 825-1110 of Human MSH6 (NP_000170).

  • Positive control

    • HepG2, Neuro2A and GL261 whole cell lysates; HeLa cells; NCIN87 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 153 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.
  • Involvement in disease

    Defects in MSH6 are the cause of hereditary non-polyposis colorectal cancer type 5 (HNPCC5) [MIM:600678]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. MSH6 mutations appear to be associated with atypical HNPCC and in particular with development of endometrial carcinoma or atypical endometrial hyperplasia, the presumed precursor of endometrial cancer. Defects in MSH6 are also found in familial colorectal cancers (suspected or incomplete HNPCC) that do not fulfill the Amsterdam criteria for HNPCC.
    Defects in MSH6 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
  • Sequence similarities

    Belongs to the DNA mismatch repair mutS family.
    Contains 1 PWWP domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA mismatch repair protein Msh6 antibody
    • G/T mismatch binding protein antibody
    • G/T mismatch-binding protein antibody
    • GTBP antibody
    • GTMBP antibody
    • hMSH6 antibody
    • HNPCC 5 antibody
    • HNPCC5 antibody
    • HSAP antibody
    • MSH 6 antibody
    • MSH6 antibody
    • MSH6_HUMAN antibody
    • mutS (E. coli) homolog 6 antibody
    • MutS alpha 160 kDa subunit antibody
    • MutS homolog 6 (E. coli) antibody
    • mutS homolog 6 antibody
    • MutS-alpha 160 kDa subunit antibody
    • p160 antibody
    • Sperm associated protein antibody
    see all

Images

  • All lanes : Anti-MSH6 antibody (ab137457) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : MSH6 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 153 kDa

  • Anti-MSH6 antibody (ab137457) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 153 kDa



    5% SDS PAGE
  • All lanes : Anti-MSH6 antibody (ab137457) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 153 kDa



    5% SDS-PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling MSH6 using ab137457 at 1/500 dilution. Right panel merged with DNA probe.
  • Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue labelling MSH6 using ab137457 at 1/100 dilution.

References

This product has been referenced in:

  • Liu S  et al. Okazaki fragment maturation involves a-segment error editing by the mammalian FEN1/MutSa functional complex. EMBO J 34:1829-43 (2015). Read more (PubMed: 25921062) »
  • Cen L  et al. Efficacy of protracted temozolomide dosing is limited in MGMT unmethylated GBM xenograft models. Neuro Oncol 15:735-46 (2013). Read more (PubMed: 23479134) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

I am assuming the species customer of tissue sections is human - antibodeis are asfollows;

The best anti annexin a1 antibody is ab47661;https://www.abcam.com/Annexin-A1-antibody-ab47661.html

anti IDH1 ab135659; https://www.abcam.com/Isocitrate-dehydrogenase-antibody-ab135659.html

anti MDM2, ab38618; https://www.abcam.com/MDM2-antibody-ab38618.html

anti PMS2, ab111253; https://www.abcam.com/PMS2-antibody-ab111253.html

ab9144; https://www.abcam.com/MLH1-antibody-ab9144.html

MSH2, ab16833; https://www.abcam.com/MSH2-antibody-ab16833.html

MSH6, ab137457; https://www.abcam.com/MSH6-antibody-ab137457.html

anti Napsin, ab73098; https://www.abcam.com/NAPSIN-A-antibody-KCG1-1-prediluted-ab73098.html

Caldesmon, ab68878; https://www.abcam.com/Caldesmon-antibody-ab68878.html

p16????? ab118459; https://www.abcam.com/p16-ARC-antibody-ab118459.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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