Overview

  • Product name

  • Description

    Rabbit polyclonal to MSI2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment within Human MSI2 (internal sequence). The exact sequence is proprietary.
    Database link: Q96DH6

  • Positive control

    • IHC-P: Mouse hindbrain and rat forebrain tissue. MCF7 xenograft. WB: MCF7 and mouse brain whole cell lysate. IHC-Fr: Adult mouse retina tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab229100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-Fr 1/100 - 1/1000.

Target

  • Function

    RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity

    Ubiquitous; detected at low levels.
  • Involvement in disease

    Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
  • Sequence similarities

    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cytoplasm. Associated with polysomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36569 antibody
    • MGC3245 antibody
    • Msi2 antibody
    • MSI2/HOXA9 fusion gene, included antibody
    • MSI2H antibody
    • MSI2H_HUMAN antibody
    • Musashi 2 antibody
    • Musashi homolog 2 antibody
    • Musashi RNA binding protein 2 antibody
    • Musashi, Drosophila, homolog of, 2 antibody
    • Musashi-2 antibody
    • RNA binding protein Musashi homolog 2 antibody
    • RNA-binding protein Musashi homolog 2 antibody
    • WD 40 repeat protein MSI2 antibody
    see all

Images

  • Anti-MSI2 antibody (ab229100) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • Frozen sectioned adult mouse retina tissue stained for MSI2 (green) using ab229100 at 1/250 dilution in immunohistochemical analysis.

    Protein kinase C alpha (red).

    Nuclear counterstain: Fluoroshield with DAPI (blue).

  • Anti-MSI2 antibody (ab229100) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded MCF7 xenograft stained for MSI2 using ab229100 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat forebrain tissue stained for MSI2 using ab229100 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse hindbrain tissue stained for MSI2 using ab229100 at 1/500 dilution in immunohistochemical analysis.

References

ab229100 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229100.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up