Recombinant
RabMAb

Recombinant Anti-MSI2 antibody [EP1305Y] (Alexa Fluor® 647) (ab199251)

Overview

  • Product name

    Anti-MSI2 antibody [EP1305Y] (Alexa Fluor® 647)
    See all MSI2 primary antibodies
  • Description

    Rabbit monoclonal [EP1305Y] to MSI2 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    A synthetic peptide derived from Human MSI2 (UniProt: Q96DH6).

  • Epitope

    Based on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2.
  • Positive control

    • ICC/IF: PC12 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab199251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • Function

    RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity

    Ubiquitous; detected at low levels.
  • Involvement in disease

    Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
  • Sequence similarities

    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cytoplasm. Associated with polysomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36569 antibody
    • MGC3245 antibody
    • Msi2 antibody
    • MSI2/HOXA9 fusion gene, included antibody
    • MSI2H antibody
    • MSI2H_HUMAN antibody
    • Musashi 2 antibody
    • Musashi homolog 2 antibody
    • Musashi RNA binding protein 2 antibody
    • Musashi, Drosophila, homolog of, 2 antibody
    • Musashi-2 antibody
    • RNA binding protein Musashi homolog 2 antibody
    • RNA-binding protein Musashi homolog 2 antibody
    • WD 40 repeat protein MSI2 antibody
    see all

Images

  • ab199251 staining MSI2 in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199251 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab199251 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199251.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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