Overview

  • Product name

    Anti-MSI2 antibody [OTI2F10]
    See all MSI2 primary antibodies
  • Description

    Mouse monoclonal [OTI2F10] to MSI2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Immunogen

    Recombinant full length protein corresponding to Human MSI2. Produced in HEK-293T cells. NP_620412
    Database link: Q96DH6

  • Positive control

    • WB: HEK-293T transfected with pCMV6-ENTRY MSI2 cDNA; HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC12 and MCF7 cell lysates. IHC-P: Human ovary adenocarcinoma, pancreas, pancreas carcinoma, endometrium adenocarcinoma and prostate tissues. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY MSI2; HeLa cells.
  • General notes

    The clone number has been updated from 2F10 to OTI2F10, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab156770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/4000. Predicted molecular weight: 35 kDa.
IHC-P 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity

    Ubiquitous; detected at low levels.
  • Involvement in disease

    Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
  • Sequence similarities

    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cytoplasm. Associated with polysomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ36569 antibody
    • MGC3245 antibody
    • Msi2 antibody
    • MSI2/HOXA9 fusion gene, included antibody
    • MSI2H antibody
    • MSI2H_HUMAN antibody
    • Musashi 2 antibody
    • Musashi homolog 2 antibody
    • Musashi RNA binding protein 2 antibody
    • Musashi, Drosophila, homolog of, 2 antibody
    • Musashi-2 antibody
    • RNA binding protein Musashi homolog 2 antibody
    • RNA-binding protein Musashi homolog 2 antibody
    • WD 40 repeat protein MSI2 antibody
    see all

Images

  • All lanes : Anti-MSI2 antibody [OTI2F10] (ab156770) at 1/200 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : SVT2 (Mouse cell line) cell lysate
    Lane 4 : A549 (Human lung carcinoma cell line) cell lysate
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
    Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 7 : MDCK (Canine kidney cell line) cell lysate
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 35 kDa

  • Immunofluorescent analysis of COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY MSI2, labeling MSI2 with ab156770 at 1/100 dilution.

  • Paraffin-embedded human ovary adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

  • Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MSI2 with ab156770 at 1/100 dilution.

  • Paraffin-embedded human pancreas tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

  • Paraffin-embedded human pancreas adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

  • Paraffin-embedded human endometrium adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

  • Paraffin-embedded human prostate tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

  • All lanes : Anti-MSI2 antibody [OTI2F10] (ab156770) at 1/4000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY MSI2 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 35 kDa

References

This product has been referenced in:

  • Choi YM  et al. DBC2/RhoBTB2 functions as a tumor suppressor protein via Musashi-2 ubiquitination in breast cancer. Oncogene 36:2802-2812 (2017). Read more (PubMed: 27941885) »
See 1 Publication for this product

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