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    msin3a-antibody-chip-grade-ab3479.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-mSin3A antibody - ChIP Grade (ab3479)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (36)

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Western blot - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • ChIP - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)

Key features and details

  • Rabbit polyclonal to mSin3A - ChIP Grade
  • Suitable for: ChIP, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-mSin3A antibody - ChIP Grade
    See all mSin3A primary antibodies
  • Description

    Rabbit polyclonal to mSin3A - ChIP Grade
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse mSin3A aa 1-19.
    Sequence:

    MKRRLDDQESDVYAAQQRR


    (Peptide available as ab4995)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • mSin3A peptide (ab4995)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ChIP
Human
ICC/IF
Mouse
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
ChIP (2)
Use at an assay dependent concentration.
ICC/IF (1)
1/50 - 1/500.
WB (5)
Use a concentration of 0.5 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 145 kDa).Can be blocked with mSin3A peptide (ab4995).
IHC-P (1)
Use a concentration of 2 µg/ml.
Notes
ChIP
Use at an assay dependent concentration.
ICC/IF
1/50 - 1/500.
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 145 kDa).Can be blocked with mSin3A peptide (ab4995).
IHC-P
Use a concentration of 2 µg/ml.

Target

  • Function

    Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.
  • Sequence similarities

    Contains 3 PAH (paired amphipathic helix) domains.
  • Post-translational
    modifications

    SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. Recruited to the nucleolus by SAP30L.
  • Target information above from: UniProt accession Q96ST3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 25942 Human
    • Entrez Gene: 20466 Mouse
    • Omim: 607776 Human
    • SwissProt: Q96ST3 Human
    • SwissProt: Q60520 Mouse
    • Unigene: 513039 Human
    • Unigene: 15755 Mouse
    • Alternative names

      • AW553200 antibody
      • DKFZP434K2235 antibody
      • FLJ90319 antibody
      • Histone deacetylase complex subunit Sin 3a antibody
      • Histone deacetylase complex subunit Sin3a antibody
      • KIAA0700 antibody
      • Kiaa4126 antibody
      • mKIAA4126 antibody
      • Paired amphipathic helix protein Sin 3a antibody
      • Paired amphipathic helix protein Sin3a antibody
      • Sin 3a antibody
      • SIN3 homolog A antibody
      • SIN3 homolog A transcription regulator (yeast) antibody
      • SIN3 homolog A transcription regulator antibody
      • SIN3 transcription regulator homolog A antibody
      • Sin3a antibody
      • SIN3A protein antibody
      • SIN3A_HUMAN antibody
      • Transcriptional co repressor Sin 3A antibody
      • Transcriptional co repressor Sin3A antibody
      • Transcriptional corepressor Sin3a antibody
      • Transcriptional regulator SIN3A antibody
      see all

    Images

    • Western blot - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Western blot - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Western blot of mSin3A on K562 cell extract using ab3479.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)

      Paraffin-embedded human ovary carcinoma tissue (right panel) stained for mSin3A using ab3479 at 1/200 dilution compared to a negative control without primary antibody (left panel) in immunohistochemical analysis, followed by HRP-conjugated secondary antibody. Detection: DAB staining.

      Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.

    • ChIP - Anti-mSin3A antibody - ChIP Grade (ab3479)
      ChIP - Anti-mSin3A antibody - ChIP Grade (ab3479)

      ChIP analysis of Sin3A was performed using cross-linked chromatin from 1x106 HCT 116 (human colorectal carcinoma cell line) cells treated with serum for 0, 15, and 30 minutes. IP was performed using a multiplex microplate Matrix ChIP assay with 1.0 µl/100 µl well volume of ab3479. Chromatin aliquots from ~1x105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µl of eluted DNA in 2 µl SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. 

    • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)

      Immunocytochemical immunoflurescence analysis of NIH-3T3 cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:100 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained with DAPI or Hoechst labelling the nuclear DNA blue. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.

    • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)

      Immunocytochemical immunoflurescence analysis of HeLa cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:200 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained blue with DAPI or Hoechst labelling the nuclear DNA and red against actin using an Alexa Fluor® 554 conjugate. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.

    • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)

      Immunocytochemical immunoflurescence analysis of NIH-3T3 cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:100 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained blue with DAPI or Hoechst labelling the nuclear DNA and red against actin using an Alexa Fluor® 554 conjugate. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)
      ab3479 (2µg/ml) staining mSin3A in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei of epithelial cells.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (36)

    Publishing research using ab3479? Please let us know so that we can cite the reference in this datasheet.

    ab3479 has been referenced in 36 publications.

    • Hwang I  et al. CIC is a critical regulator of neuronal differentiation. JCI Insight 5:N/A (2020). PubMed: 32229723
    • Pinto DO  et al. Effect of transcription inhibition and generation of suppressive viral non-coding RNAs. Retrovirology 16:13 (2019). PubMed: 31036006
    • Fuller NO  et al. CoREST Complex-Selective Histone Deacetylase Inhibitors Show Prosynaptic Effects and an Improved Safety Profile To Enable Treatment of Synaptopathies. ACS Chem Neurosci 10:1729-1743 (2019). PubMed: 30496686
    • Zhu J  et al. NKX2-8 deletion-induced reprogramming of fatty acid metabolism confers chemoresistance in epithelial ovarian cancer. EBioMedicine 43:238-252 (2019). PubMed: 31047858
    • Meier-Soelch J  et al. RNAi-Based Identification of Gene-Specific Nuclear Cofactor Networks Regulating Interleukin-1 Target Genes. Front Immunol 9:775 (2018). PubMed: 29755455
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 10 Abreviews or Q&A

    ChIP abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Tissue lysate - whole (Mouse brain postnatal day 1)
    Negative control
    Input genomic DNA processed in parallel to the mSIN3A ChIP sample and analyzed for target promoter enrichment.
    Specification
    Mouse brain postnatal day 1
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Positive control
    Our mSIN3A ChIP pull down sample analyzed for target promoter enrichment.
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 23 2020

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (Embryonic stem cell)
    Gel Running Conditions
    Non-reduced Denaturing (7%)
    Loading amount
    60 µg
    Specification
    Embryonic stem cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 19 2016

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (7%gel)
    Sample
    Mouse Tissue lysate - whole (hippocampus)
    Specification
    hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 30 2015

    Immunoprecipitation abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Specification
    HEK293T
    Total protein in input
    40 µg
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 05 2013

    ChIP abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Semiquantitative PCR
    Sample
    Human Cell lysate - nuclear (MCF7)
    Specification
    MCF7
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: FORMALDEHYDE 10%
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 05 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate
    Sample
    Mouse Tissue sections (BREAST CANCER TISSUE)
    Specification
    BREAST CANCER TISSUE
    Permeabilization
    Yes - xylene
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 05 2013

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    1e+006 cells
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 04 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH/3T3)
    Specification
    NIH/3T3
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mrs. Filomena Ricciardi

    Verified customer

    Submitted Jun 26 2012

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (THP-1 cells)
    Loading amount
    10 µg
    Specification
    THP-1 cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 21 2007

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (Lung)
    Loading amount
    10 µg
    Specification
    Lung
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 21 2007

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