Key features and details
- Rabbit polyclonal to MSK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MSK1 antibody
See all MSK1 primary antibodies
DescriptionRabbit polyclonal to MSK1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human MSK1 aa 551-600 (internal sequence).
Database link: O75582
- Human brain cortex neurons tissue, Human heart tissue, 293 cells, Huvec cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 48% PBS, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 90 kDa.
Please Note: ab155405 has only been tested in lysates from cells which have undergone stress.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionSerine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
Tissue specificityWidely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsSer-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 5 antibody
- EC 184.108.40.206 antibody
- KS6A5_HUMAN antibody
All lanes : Anti-MSK1 antibody (ab155405) at 1/500 dilution
Lane 1 : 293 cells treated with UV for 15 minutes
Lane 2 : 293 cells treated with UV for 15 minutes with synthesised peptide
Predicted band size: 90 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex neurons tissue labeling MSK1 using ab155405 at 5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue labeling MSK1 using ab155405 at 5 µg/ml.
Immunofluorescence analysis of Huvec cells, labeling MSK1 using ab155405 at a 1/100 dilution. The image on the right is treated with the synthesized peptide.
ab155405 has not yet been referenced specifically in any publications.