Product nameAnti-MSK1 (phospho S212) antibody
See all MSK1 primary antibodies
DescriptionRabbit polyclonal to MSK1 (phospho S212)
Specificityab79499 detects endogenous levels of MSK1 only when phosphorylated at serine 212
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of serine 212 (AYSPFC)
- IHC: human breast carcinoma tissue WB: extracts from 3T3 cells (treated with EGF at 200 ng/ml for 5mins)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by affinity chromatography using an epitope specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 90 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionSerine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
Tissue specificityWidely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsSer-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 5 antibody
- EC 18.104.22.168 antibody
- KS6A5_HUMAN antibody
All lanes : Anti-MSK1 (phospho S212) antibody (ab79499) at 1/1000 dilution
Lane 1 : extracts from 3T3 cells (treated
with EGF at 200ng/ml for 5 mins)
Lane 2 : extracts from 3T3 cells (treated
with EGF at 200ng/ml for 5 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, stained with ab79499, at 1/100 dilution, in the absence and presence of the immunising peptide
ab79499 has not yet been referenced specifically in any publications.