RecombinantRabMAb

Recombinant Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)

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Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
  • Immunocytochemistry - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
  • Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
  • Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
  • Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E375] to MSK1 (phospho S376) - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free
    See all MSK1 primary antibodies

  • Description

    Rabbit monoclonal [E375] to MSK1 (phospho S376) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody will detect MSK1 phosphorylation on Serine 376.

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Human MSK1. The exact sequence is proprietary.

  • Positive control

    • WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma. ICC: HepG2 cells

  • General notes

    ab210529 is the carrier-free version of ab32190 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab210529 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab210529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

    • Tissue specificity

      Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.

    • Post-translational
      modifications

      Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.

    • Cellular localization

      Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
    • Information by UniProt

    • Database links

      • Alternative names

        • 90 kDa ribosomal protein S6 kinase 5 antibody
        • EC 2.7.11.1 antibody
        • KS6A5_HUMAN antibody
        • MGC1911 antibody
        • Mitogen and stress activated protein kinase 1 antibody
        • MSPK1 antibody
        • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
        • Nuclear mitogen- and stress-activated protein kinase 1 antibody
        • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
        • Ribosomal protein S6 kinase 90kDa antibody
        • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
        • Ribosomal Protein S6 Kinase Alpha 5 antibody
        • Ribosomal protein S6 kinase alpha-5 antibody
        • RLPK antibody
        • RPS6KA5 antibody
        • RSK Like Protein Kinase antibody
        • RSK-like protein kinase antibody
        • RSKL antibody
        • S6K alpha 5 antibody
        • S6K-alpha-5 antibody
        see all

      Images

      • Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

        All lanes : HEK-293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 90 kDa


        Exposure time: 10 seconds


        This data was developed using ab32190, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

        Observed bands: 90, 80 kDa.

        The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

      • Immunocytochemistry - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        Immunocytochemistry - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)

        Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.

        Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37℃ 1h).

        This data was developed using ab32190, the same antibody clone in a different buffer formulation.

         

      • Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

        Lane 1 : Untreated HEK293 (human embryonic kidney) whole cell lysate
        Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate
        Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate. Then the membrane was incubated with Alkaline phosphatase.

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 90 kDa


        Exposure time: 5 seconds


        This data was developed using ab32190, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

        Observed band: 90, 80 kDa.

        The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

      • Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution

        Lane 1 : Untreated K562 cell lysate
        Lane 2 : K562 cell lysate treated with Alkaline

        Predicted band size: 90 kDa
        Observed band size: 87 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab32190, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        This data was developed using ab32190, the same antibody clone in a different buffer formulation.Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
        Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab210529? Please let us know so that we can cite the reference in this datasheet.

      ab210529 has not yet been referenced specifically in any publications.

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