Recombinant
RabMAb

Recombinant Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)

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Overview

  • Product name

    Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free
    See all MSK1 primary antibodies

  • Description

    Rabbit monoclonal [E375] to MSK1 (phospho S376) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody will detect MSK1 phosphorylation on Serine 376.

  • Tested applications

    Suitable for: IP, IHC-P, ICC, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human MSK1. The exact sequence is proprietary.

  • Positive control

    • WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma.

  • General notes

    ab210529 is the carrier-free version of ab32190 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab210529 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab210529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

    • Tissue specificity

      Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.

    • Post-translational
      modifications

      Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.

    • Cellular localization

      Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
    • Information by UniProt

    • Database links

      • Alternative names

        • 90 kDa ribosomal protein S6 kinase 5 antibody
        • EC 2.7.11.1 antibody
        • KS6A5_HUMAN antibody
        • MGC1911 antibody
        • Mitogen and stress activated protein kinase 1 antibody
        • MSPK1 antibody
        • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
        • Nuclear mitogen- and stress-activated protein kinase 1 antibody
        • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
        • Ribosomal protein S6 kinase 90kDa antibody
        • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
        • Ribosomal Protein S6 Kinase Alpha 5 antibody
        • Ribosomal protein S6 kinase alpha-5 antibody
        • RLPK antibody
        • RPS6KA5 antibody
        • RSK Like Protein Kinase antibody
        • RSK-like protein kinase antibody
        • RSKL antibody
        • S6K alpha 5 antibody
        • S6K-alpha-5 antibody
        see all

      References

      ab210529 has not yet been referenced specifically in any publications.

      Publishing research using ab210529? Please let us know so that we can cite the reference in this datasheet.

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