Product nameAnti-MSK2 / RSK-B antibody
See all MSK2 / RSK-B primary antibodies
DescriptionRabbit polyclonal to MSK2 / RSK-B
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide, corresponding to a region between residues 722 - 772 of Human MSK2/ RSK-B (NP_003933.1).
- HeLa, 293T or NIH 3T3 cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 86 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 4 antibody
- EC 220.127.116.11 antibody
- KS6A4_HUMAN antibody
All lanes : Anti-MSK2 / RSK-B antibody (ab99411) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MSK2 / RSK-B with ab99411 at 1/200 (1µg/mg). Detection: DAB.
Detection of MSK2 / RSK-B in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab99411 at 3 µg/mg lysate for IP, and at 1 µg/ml for the subsequent western blot.
Detection: chemiluminescence with an exposure time of 30 seconds.
ab99411 has been referenced in 1 publication.
- Khan P et al. Mitogen- and stress-activated protein kinases 1 and 2 are required for maximal trefoil factor 1 induction. PLoS One 8:e63189 (2013). PubMed: 23675462