Overview

  • Product name

    Anti-MSP-1 antibody
  • Description

    Rabbit polyclonal to MSP-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Arabidopsis thaliana, Tobacco, Wheat
  • Immunogen

    Recombinant full length protein corresponding to MSP-1.

  • Positive control

    • Arabidopsis and tobacco chloroplast proteins and Synechocystis sp. PCC 6803 membrane proteins.
  • General notes

     This product was previously labelled as psbO

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 30 kDa.

1/1000 (for cyanobacteria) - 1/3000 (for higher plants).

Target

  • Relevance

    psbO is an extrinsic subunit of photosystem II and has been proposed to play a central role in stabilisation of the catalytic manganese cluster. Arabidopsis genome encodes two psbO genes: psbO1 and psbO2. PsbO1 protein is the major isoform in the wild type.
  • Cellular localization

    Plastid, chloroplast thylakoid membrane
  • Database links

    • Alternative names

      • 33 kDa subunit of oxygen evolving system of photosystem II antibody
      • 33 kDa thylakoid membrane protein antibody
      • OEC 33 kDa subunit antibody
      • OEE1 antibody
      • Oxygen evolving enhancer protein 1-1, chloroplast antibody
      • Oxygen evolving enhancer protein 1-2, chloroplast antibody
      • Photosystem II manganese stabilizing polypeptide antibody
      • PSBO1 antibody
      • PSBO2 antibody
      see all

    Images

    • All lanes : Anti-MSP-1 antibody (ab65563)

      Lane 1 : Arabidopsis thaliana chloroplast proteins
      Lane 2 : Nicotiana tabaccum chloroplast proteins

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Anti-rabbit at 1/30000 dilution

      Predicted band size: 30 kDa



      At – Arabidopsis thaliana chloroplast proteins Nt – Nicotiana tabaccum chloroplast proteins At – Arabidopsis thaliana chloroplast proteins
      Nt – Nicotiana tabaccum chloroplast proteins

    References

    This product has been referenced in:

    • Henry E  et al. Beyond glycolysis: GAPDHs are multi-functional enzymes involved in regulation of ROS, autophagy, and plant immune responses. PLoS Genet 11:e1005199 (2015). WB ; Arabidopsis thaliana . Read more (PubMed: 25918875) »
    • Zhang L  et al. Deletion of Synechocystis sp. PCC 6803 leader peptidase LepB1 affects photosynthetic complexes and respiration. Mol Cell Proteomics 12:1192-203 (2013). Read more (PubMed: 23358502) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us and sorry for the delay of our reply. The source of the antibody ab65563 informed me that : in the Western Blot experiments PsbO is also running with Molecular weight of 35 kDa. It has probably something to do with the migration behaviour of the protein. If one compare the migration of PsbO from higher plants and Synechocystis, the latter protein is migrating still slightly lower as higher plant protein which has MW of 33 kDa. The antibodies were tested many times in different PsbO deficient mutants and we are not doubting of the quality of these antibodies. We have also compared the migration of PsbO with different independetly produced antibodies and the picture was always the same. Yes, we also see very week band around 47 - 50 kDa but it is at least 10 times weaker as PsbO band. This is probably the unspecific reaction which can be ignored. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

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