Recombinant Anti-MSP/MST1 antibody [EPR22184-180] (ab245190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22184-180] to MSP/MST1
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-MSP/MST1 antibody [EPR22184-180]
See all MSP/MST1 primary antibodies -
Description
Rabbit monoclonal [EPR22184-180] to MSP/MST1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse MSP/MST1 active protein (aa1-716); Mouse serum.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22184-180 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 81,60 kDa (predicted molecular weight: 81 kDa).
|
Notes |
---|
WB
1/1000. Detects a band of approximately 81,60 kDa (predicted molecular weight: 81 kDa). |
Target
-
Function
Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsMay be cleaved after Arg-483, to yield two chains held together by disulfide bonds, or two separate polypeptides. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 4485 Human
- Entrez Gene: 15235 Mouse
- Omim: 142408 Human
- SwissProt: P26927 Human
- SwissProt: P26928 Mouse
- Unigene: 349110 Human
- Unigene: 512587 Human
- Unigene: 8369 Mouse
-
Alternative names
- D3F15S2 antibody
- DNF15S2 antibody
- Hepatocyte growth factor like protein alpha chain antibody
see all
Images
-
All lanes : Anti-MSP/MST1 antibody [EPR22184-180] (ab245190) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Observed band size: 60, 80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Pro-MSP is proteolytically cleaved into alpha and beta chain. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8393443;8609228;7732008;11856768).
-
Anti-MSP/MST1 antibody [EPR22184-180] (ab245190) at 1/1000 dilution + Mouse MSP/MST1 active protein 20 ng (aa1-716)
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Exposure time: 6 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The positive band (~60-kDa) is the MSP alpha chain.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab245190 has been referenced in 1 publication.
- Wang Y et al. A Global Phosphorylation Atlas of Proteins Within Pathological Site of Rotator Cuff Tendinopathy. Front Mol Biosci 8:787008 (2021). PubMed: 35242811