Product nameAnti-MSP/MST1 antibody [EPR22184-180] - BSA and Azide free
See all MSP/MST1 primary antibodies
DescriptionRabbit monoclonal [EPR22184-180] to MSP/MST1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-Fr,IHC-P or IP
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse MSP/MST1 aa 1-500. The exact sequence is proprietary.
Database link: P26928
- WB: Mouse serum.
Ab245826 is the carrier-free version of ab245190. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab245826 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab245826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 81, 60 kDa (predicted molecular weight: 81 kDa).|
FunctionProbably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved.
Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain.
modificationsMay be cleaved after Arg-483, to yield two chains held together by disulfide bonds, or two separate polypeptides.
- Information by UniProt
- D3F15S2 antibody
- DNF15S2 antibody
- Hepatocyte growth factor like protein alpha chain antibody
All lanes : Anti-MSP/MST1 antibody [EPR22184-180] (ab245190) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Observed band size: 60, 80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
Pro-MSP is proteolytically cleaved into alpha and beta chain. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8393443;8609228;7732008;11856768).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245190).
ab245826 has not yet been referenced specifically in any publications.