Anti-MSRA antibody (ab232901)
Key features and details
- Rabbit polyclonal to MSRA
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-MSRA antibody
See all MSRA primary antibodies -
Description
Rabbit polyclonal to MSRA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Cow -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human MSRA aa 1 to the C-terminus. (Expressed in E.coli).
Database link: Q9UJ68 -
Positive control
- WB: Recombinant human MSRA protein; HT1080 and HepG2 cell lysates; Pig and rat kidney lysates. IHC-P: Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232901 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 26 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 26 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. -
Tissue specificity
Ubiquitous. Highest expression in adult kidney and cerebellum, followed by liver, heart ventricles, bone marrow and hippocampus. -
Sequence similarities
Belongs to the MsrA Met sulfoxide reductase family. -
Cellular localization
Cytoplasm; Cytoplasm. Nucleus and Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 281312 Cow
- Entrez Gene: 4482 Human
- Entrez Gene: 100152960 Pig
- Entrez Gene: 29447 Rat
- Omim: 601250 Human
- SwissProt: P54149 Cow
- SwissProt: Q9UJ68 Human
- SwissProt: Q923M1 Rat
see all -
Alternative names
- Cytosolic methionine S sulfoxide reductase antibody
- Methionine sulphoxide reductase A antibody
- Mitochondrial peptide methionine sulfoxide reductase antibody
see all
Images
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Anti-MSRA antibody (ab232901) at 2 µg/ml + Recombinant human MSRA protein
Predicted band size: 26 kDa -
Anti-MSRA antibody (ab232901) at 2 µg/ml + HT1080 (human fibrosarcoma cell line) cell lysate
Predicted band size: 26 kDa -
Anti-MSRA antibody (ab232901) at 2 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 26 kDa -
Anti-MSRA antibody (ab232901) at 2 µg/ml + Pig kidney lysate
Predicted band size: 26 kDa -
Anti-MSRA antibody (ab232901) at 3 µg/ml + Rat kidney lysate
Predicted band size: 26 kDa -
Formalin-fixed, paraffin-embedded human brain tissue stained for MSRA using ab232901 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232901 has not yet been referenced specifically in any publications.