Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13664] to MSRB3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-MSRB3 antibody [EPR13664]
See all MSRB3 primary antibodies
DescriptionRabbit monoclonal [EPR13664] to MSRB3
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human MSRB3 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q8IXL7
- Human fetal heart tissue lysate, Human colon cancer tissue lysate, A549 cell line lysate, HeLa cell line lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab180584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).|
FunctionCatalyzes the reduction of free and protein-bound methionine sulfoxide to methionine. Isoform 2 is essential for hearing.
Tissue specificityWidely expressed.
Involvement in diseaseDeafness, autosomal recessive, 74
Sequence similaritiesBelongs to the MsrB Met sulfoxide reductase family.
Contains 1 MsrB (methionine-R-sulfoxide reductase) domain.
Cellular localizationMitochondrion and Endoplasmic reticulum.
- Information by UniProt
- Deafness, Autosomal Recessive 74 antibody
- DFNB74 antibody
- FLJ36866 antibody
All lanes : Anti-MSRB3 antibody [EPR13664] (ab180584) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human colon tissue lysate
Lane 3 : A549 cell line lysate
Lane 4 : HeLa cell line lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
ab180584 has been referenced in 1 publication.
- Zhang LC et al. mRNA and protein expression levels of four candidate genes for ear size in Erhualian and Large White pigs. Genet Mol Res 16:N/A (2017). PubMed: 28407177