Anti-MST antibody (ab154514)
Key features and details
- Rabbit polyclonal to MST
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-MST antibody
See all MST primary antibodies -
Description
Rabbit polyclonal to MST -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human MST aa 200-300 (C terminal).
Database link: P25325 -
Positive control
- Recombinant Human MST protein (ab114832) can be used as a positive control in WB. WB: A431 cells. Mouse tissue extract. IHC-P: CL1-5 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
Target
-
Function
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis. -
Sequence similarities
Contains 2 rhodanese domains. -
Domain
The structure consists of 2 domains of very similar conformation, suggesting a common evolutionary origin. However, the sequences of the 2 domains are very different. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 4357 Human
- Entrez Gene: 246221 Mouse
- Omim: 602496 Human
- SwissProt: P25325 Human
- SwissProt: Q99J99 Mouse
- Unigene: 248267 Human
- Unigene: 294215 Mouse
-
Alternative names
- 3 mercaptopyruvate sulfurtransferase antibody
- 3-mercaptopyruvate sulfurtransferase antibody
- Human liver rhodanese antibody
see all
Images
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded CL1-5 xenograft labeling MST with ab154514 at 1/100.
-
Anti-MST antibody (ab154514) at 1/500 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 33 kDa12% SDS PAGE
-
Anti-MST antibody (ab154514) at 1/500 dilution + Mouse tissue extract at 50 µg
Predicted band size: 33 kDa12% SDS-PAGE
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab154514 has been referenced in 2 publications.
- Pal VK et al. Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis. Elife 10:N/A (2021). PubMed: 34792020
- Panagaki T et al. Role of Hydrogen Sulfide and 3-Mercaptopyruvate Sulfurtransferase in the Regulation of the Endoplasmic Reticulum Stress Response in Hepatocytes. Biomolecules 10:N/A (2020). PubMed: 33352938